Induction of the fatty acid 2-hydroxylase (FA2H) gene by Δ9-tetrahydrocannabinol in human breast cancer cells
Induction of the fatty acid 2-hydroxylase (FA2H) gene by Δ9-tetrahydrocannabinol in human breast cancer cells
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DOI：
10.2131/jts.38.305
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发表时间：
2013-04-01
影响因子：
2
通讯作者：
Aramaki, Hironori
中科院分区：
文献类型：
--
作者：
Takeda, Shuso;Harada, Mari;Aramaki, Hironori
为了研究受Delta（9）-四氢大麻酚（Delta（9）-THC）调节的基因，我们对人乳腺癌MDA-MB-231细胞进行了DNA微阵列分析，该细胞是低分化的乳腺癌细胞，用Delta（9）-THC以约25 μ M的IC 50浓度处理48小时。在观察到的高度上调的基因（> 10倍）中，脂肪酸2-羟化酶（FA 2 H）被显著诱导（17.8倍）。虽然FA 2 H的生理作用尚未完全了解，但FA 2 H已显示出调节细胞分化。Delta（9）-THC暴露后油红0染色的结果显示细胞中脂滴的分布（分化表型的标志）。总之，这里获得的结果表明FA 2 H是一种新的Delta（9）-THC调节的基因，并且Delta（9）-THC在低分化的MDA-MB-231细胞中诱导分化信号。
To investigate gene(s) being regulated by Delta(9)-tetrahydrocannabinol (Delta(9)-THC), we performed DNA microarray analysis of human breast cancer MDA-MB-231 cells, which are poorly differentiated breast cancer cells, treated with Delta(9)-THC for 48 hr at an IC50 concentration of approximately 25 mu M. Among the highly up-regulated genes (> 10-fold) observed, fatty acid 2-hydroxylase (FA2H) was significantly induced (17.8-fold). Although the physiological role of FA2H has not yet been fully understood, FA2H has been shown to modulate cell differentiation. The results of Oil Red 0 staining after Delta(9)-THC exposure showed the distribution of lipid droplets (a sign of the differentiated phenotype) in cells. Taken together, the results obtained here indicate that FA2H is a novel Delta(9)-THC-regulated gene, and that Delta(9)-THC induces differentiation signal(s) in poorly differentiated MDA-MB-231 cells.