Rice DWARF AND LOW-TILLERING and the homeodomain protein OSH15 interact to regulate internode elongation via orchestrating brassinosteroid signaling and metabolism.
Rice DWARF AND LOW-TILLERING and the homeodomain protein OSH15 interact to regulate internode elongation via orchestrating brassinosteroid signaling and metabolism.
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水稻矮化和低分蘖与同源域蛋白 OSH15 相互作用，通过协调油菜素类固醇信号和代谢来调节节间伸长
DOI：
10.1093/plcell/koac196
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发表时间：
2022-09-27
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胸腺类固醇（BR）植物激素在调节大米（Oryza sativa）的节节性伸长率中起着至关重要的作用。但是，基本机制仍然在很大程度上不清楚。矮人和低浸泡（DLT）突变体是一种轻度的BR信号缺陷突变体。在这里，我们确定了两个DLT增强剂，这些增强剂显示出与最高节间（IN1）相比，较低节间的更严重缩短。这两个突变体都携带Oryza sativa homeobox 15（OSH15）的等位基因，这是矮人6型突变体的创建基因，它们缩短了较低的节间，但不缩短1个。与突变表型一致，OSH15表达在较低的节间，尤其是在In2中，而不是In1。 OSH15单个突变体损害了BR敏感性，并伴随着幼苗中的BR合成增强。 DLT与OSH15物理相互作用，以在幼苗和节间中共同调节许多基因。 OSH15靶向并促进BR受体基因BR不敏感1（OSBRI1）的表达，并以剂量依赖性方式促进了这种调节。在OSH15，DLT和OSH15 DLT中，幼苗和圆锥花序中的BR水平较高，但与野生型相比，节间的突发性较低。综上所述，我们的结果表明，DLT与在较低节间发挥作用的OSH15相互作用，以通过编排BR信号传导和代谢来调节水稻节节性伸长。
矮和低浸泡与同源域蛋白OSH15相互作用，后者蛋白OSH15直接靶向甘油类固醇受体基因OSBRI1，并在较低的节间中表达，以调节胸前固醇信号和代谢来调节节节节延伸。
Brassinosteroid (BR) phytohormones play crucial roles in regulating internode elongation in rice (Oryza sativa). However, the underlying mechanism remains largely unclear. The dwarf and low-tillering (dlt) mutant is a mild BR-signaling-defective mutant. Here, we identify two dlt enhancers that show more severe shortening of the lower internodes compared to the uppermost internode (IN1). Both mutants carry alleles of ORYZA SATIVA HOMEOBOX 15 (OSH15), the founding gene for dwarf6-type mutants, which have shortened lower internodes but not IN1. Consistent with the mutant phenotype, OSH15 expression is much stronger in lower internodes, particularly in IN2, than IN1. The osh15 single mutants have impaired BR sensitivity accompanied by enhanced BR synthesis in seedlings. DLT physically interacts with OSH15 to co-regulate many genes in seedlings and internodes. OSH15 targets and promotes the expression of the BR receptor gene BR INSENSITIVE1 (OsBRI1), and DLT facilitates this regulation in a dosage-dependent manner. In osh15, dlt, and osh15 dlt, BR levels are higher in seedlings and panicles, but unexpectedly lower in internodes compared with the wild-type. Taken together, our results suggest that DLT interacts with OSH15, which functions in the lower internodes, to modulate rice internode elongation via orchestrating BR signaling and metabolism. DWARF AND LOW-TILLERING interacts with the homeodomain protein OSH15, which directly targets the brassinosteroid receptor gene OsBRI1 and is expressed in lower internodes, to regulate the internode elongation via modulating brassinosteroid signaling and metabolism.
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影响因子：
5.1
作者：
Bart R;Chern M;Park CJ;Bartley L;Ronald PC
通讯作者：
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使用下一代 DNA 测序数据进行变异发现和基因分型的框架。
影响因子：
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