Inhaled leukotriene E4, but not leukotriene D4, increased airway inflammatory cells in subjects with atopic asthma
Inhaled leukotriene E4, but not leukotriene D4, increased airway inflammatory cells in subjects with atopic asthma
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DOI：
10.1164/ajrccm.164.8.2102033
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发表时间：
2001-10-15
影响因子：
24.7
通讯作者：
O'Byrne, PM
中科院分区：
文献类型：
--
作者：
Gauvreau, GM;Parameswaran, KN;O'Byrne, PM
过敏原诱导的晚期气道反应与气道嗜酸性粒细胞和嗜碱性粒细胞数量增加有关。本研究的目的是比较和对比吸入半胱氨酰白三烯LTD 4和LTE 4，这是在过敏原诱导的气道反应，过敏原，气道炎症细胞释放的影响。15例特应性轻度哮喘受试者吸入稀释剂、LTD 4、LTE 4和过敏原。肺量测定进行了7小时，并测量痰液炎性细胞前，7小时，24小时后的挑战。在LTD 4、LTE 4、过敏原和稀释剂之后，FEV 1的最大早期百分比下降分别为23.6 +/-1.4%、21.6 +/-2.3%、29.3 +/-2.4%和4.0 +/- 1.1%。仅吸入LTE 4和过敏原后7 h和24 h痰嗜酸性粒细胞显著增加，7 h痰嗜碱性粒细胞显著增加。另外6名受试者在吸入后4小时进行气道活检。与LTD 4和稀释剂相比，吸入LTE 4后固有层中有显著更多的嗜酸性粒细胞（p < 0.05）。这些结果表明，在过敏性哮喘中，半胱氨酰白三烯在嗜酸性粒细胞迁移到气道中起作用，并且对于相同程度的支气管收缩，吸入LTE 4比LTD 4引起更多的组织和气道嗜酸性粒细胞增多。
Allergen-induced late airway responses are associated with increased numbers of airway eosinophils and basophils. The purpose of this study was to compare and contrast the effects of inhaled cysteinyl leukotrienes LTD4 and LTE4, which are released during allergen-induced airway responses, and allergen, on airway inflammatory cells. Fifteen subjects with atopic, mild asthma inhaled diluent, LTD4, LTE4, and allergen. Spirometry was performed for 7 h, and sputum inflammatory cells were measured before, 7 h, and 24 h after challenges. The maximum early percent fall in FEV1 was 23.6 +/- 1.4%, 21.6 +/- 2.3%, 29.3 +/- 2.4%, and 4.0 +/- 1.1% after LTD4, LTE4, allergen, and diluent, respectively. Only inhaled LTE4 and allergen significantly increased sputum eosinophils at 7 h and 24 h, and sputum basophils at 7 h. Six additional subjects underwent airway biopsies 4 h after inhalation. There were significantly more eosinophils in the lamina propria after inhalation of LTE4 compared with LTD4 and diluent (p < 0.05). These results suggest cysteinyl leukotrienes play a role in eosinophil migration into the airways in allergic asthma, and for the same degree of bronchoconstriction, inhaled LTE4 causes more tissue and airway eosinophilia than LTD4.