SYSTEMS-THEORY APPROACH TO THE ANALYSIS OF MULTI-EXPONENTIAL FLUORESCENCE DECAY
SYSTEMS-THEORY APPROACH TO THE ANALYSIS OF MULTI-EXPONENTIAL FLUORESCENCE DECAY
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DOI：
10.1016/s0006-3495(79)85236-4
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发表时间：
1979-01-01
影响因子：
3.4
通讯作者：
FORD, CC
中科院分区：
文献类型：
--
作者：
EISENFELD, J;FORD, CC
提出了基于线性系统理论的荧光衰减实验的数学模型。该模型提出了一种实验技术，可以增加正确确定多组分系统的衰减常数的可能性。使用矩量法进行数据分析，通过结合从多个离散实验获得的信息来提高准确性。示例表明，随着实验测定次数从 1 增加到 4，由已知标准品 [芘、咔唑和 2,5-二苯基恶唑] 组成的 3 组分系统的分析得到改善。通过改变激发和发射波长进行离散测量。
A mathematical model of the fluorescence decay experiment based on linear systems theory is presented. The model suggests an experimental technique that increases the probability of correctly determining the decay constants of a multicomponent system. Ths use of moment methods for data analysis improves accuracy by combining information obtained from several discrete experiments. Examples are presented to show that the analysis of a 3-component system composed of known standards [pyrene, carbazole and 2,5-diphenyloxazole] is improved as the number of experimental determinations is increased fron 1 to 4. The discrete measurements are made by changing the excitation and emission wavelengths.