Vitreous levels of stromal cell-derived factor 1 and vascular endothelial growth factor in patients with retinopathy of prematurity
Vitreous levels of stromal cell-derived factor 1 and vascular endothelial growth factor in patients with retinopathy of prematurity
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DOI：
10.1016/j.ophtha.2007.08.050
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发表时间：
2008-06-01
期刊：
影响因子：
13.7
通讯作者：
Trese, Michael T.
中科院分区：
文献类型：
--
作者：
Sonmez, Kenan;Drenser, Kimberly A.;Trese, Michael T.
目的：进一步了解血管内皮生长因子（VEGF）和基质细胞衍生因子1 α (SDF-1 α)在早产儿视网膜病变（ROP）发病机制中的作用。设计：实验研究。参与者：研究组包括22只4期ROP眼（12只血管活跃，10只血管不活跃），从中获得玻璃体样本。5只接受先天性白内障手术的眼的玻璃体样本作为对照。方法：分析玻璃体样品中总蛋白、VEGF和SDIF-1 α的浓度。手术时对血管活动进行分级，并定义为存在附加疾病，新血管生长到嵴或牵拉性脱离区域的玻璃体上，或积液性和牵拉性视网膜脱离合并。主要观察指标：比较血管活跃的4期ROP眼、血管不活跃的4期ROP眼和对照眼玻璃体中VEGF和SDF-1 α的浓度。结果：ROP组玻璃体中VEGF和SDF-1 α浓度明显高于对照组。对照组中位VEGF水平为59 pg/ml（范围38-135 pg/ml），血管无活性ROP组中位VEGF水平为316 pg/ml（范围105-665 pg/ml），血管活性ROP组中位VEGF水平为3454 pg/ml（范围774-8882 pg/ml）。对照组中位SDF-1 α水平为327 pg/ml（范围299-393 pg/ml），血管非活动性ROP组为609 pg/ml（范围515-1116 pg/ml），血管活动性ROP组为1029 pg/ml（范围807-3015 pg/ml）。三组间玻璃体VEGF、SDF-1 α浓度差异均有统计学意义(P
Purpose: To understand better the role of vascular endothelial growth factor (VEGF) and stromal cell-derived factor 1 alpha (SDF-1 alpha) in the pathogenesis of retinopathy of prematurity (ROP).Design: Experimental study.Participants: The study group consisted of 22 eyes with stage 4 ROP (12 vascularly active, 10 vascularly inactive), from which vitreous samples were obtained. Vitreous samples from 5 eyes undergoing surgery for congenital cataract were used as controls.Methods: The vitreous samples were analyzed for concentrations of total protein, VEGF, and SDIF-1 alpha. Vascular activity was graded at the time of surgery and was defined as the presence of plus disease, neovascularization growing onto the vitreous at the ridge or tractional detachment area, or combined effusive and tractional retinal detachment. Main OutcomeMeasures: Vitreous concentrations of VEGF and SDF-1 alpha were compared among vascularly active stage 4 ROP eyes, vascularly inactive stage 4 ROP eyes, and control eyes.Results: Vitreous concentrations of VEGF and SDF-1 alpha were elevated in eyes with ROP compared with control eyes. The median VEGF level was 59 pg/ml (range, 38-135 pg/ml) in the control group, 316 pg/ml (range, 105-665 pg/ml) in the vascularly inactive ROP group, and 3454 pg/ml (range, 774-8882 pg/ml) in the vascularly active ROP group. The median SDF-1 alpha level was 327 pg/ml (range, 299-393 pg/ml) in the control group, 609 pg/ml (range, 515-1116 pg/ml) in the vascularly inactive ROP group, and 1029 pg/ml (range, 807-3015 pg/ml) in the vascularly active ROP group. The differences in both vitreous VEGF and SDF-1 alpha concentrations between the three groups were statistically significant (P