MAC-1 - MACROPHAGE DIFFERENTIATION ANTIGEN IDENTIFIED BY MONOCLONAL ANTIBODY
MAC-1 - MACROPHAGE DIFFERENTIATION ANTIGEN IDENTIFIED BY MONOCLONAL ANTIBODY
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DOI：
10.1002/eji.1830090410
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发表时间：
1979-01-01
影响因子：
5.4
通讯作者：
MILSTEIN, C
中科院分区：
文献类型：
--
作者：
SPRINGER, T;GALFRE, G;MILSTEIN, C
M1/70是一种分泌单克隆大鼠抗小鼠细胞表面抗体的杂交骨髓瘤细胞系。采用125i标记的抗大鼠Ig[免疫球蛋白]G间接结合法、荧光活化细胞分选仪、放射自显影法和细胞表面分子沉淀法研究该抗原的细胞分布。肿瘤细胞组筛选[S1A， BW5147， TIMI， P1798和R8CL7淋巴瘤]显示与巨噬细胞样细胞系有很强的反应性，但与B[骨髓来源]或T[胸腺来源]淋巴瘤细胞系无反应性。在正常组织中，M1/70抗原少量存在于脾脏和渗出粒细胞和骨髓细胞亚群中，适量存在于脾脏和血液单核细胞中，大量表达于脾脏组织细胞和腹膜渗出巨噬细胞中。红细胞和淋巴细胞中均不存在M1/70抗原，M1/70抗体在腹膜渗出巨噬细胞中析出190,000和105,000 MW的2个多肽，其含量远高于脾细胞。同时检测了经单克隆抗体M1/69和M1/9.3鉴定的另外2种抗原在吞噬细胞上的表达。单核细胞和粒细胞大量表达M1/69抗原，少量表达M1/70抗原；在腹膜渗出巨噬细胞中，这种模式被戏剧性地逆转。M1/70因此定义了一种在单核吞噬细胞和粒细胞上的分化抗原，其表达在单核细胞成熟过程中特异性增加。这种抗体是第一个被描述的识别特定于吞噬细胞的离散分子的抗体。
The derivation of M1/70, a hybrid myeloma line secreting monoclonal rat anti-mouse cell surface antibody, was previously described. The cellular distribution of this antigen was investigated using a 125I-labeled anti-rat Ig[immunoglobulin]G indirect binding assay, the fluorescence-activated cell sorter, autoradiography and precipitation of cell surface molecules. Screening with a tumor cell panel [S1A, BW5147, TIMI, P1798 and R8CL7 lymphomas] showed strong reactivity with a macrophage-like line but no reactivity with B [bone marrow-derived] or T [thymus-derived] lymphoma lines. In normal tissues, M1/70 antigen was present in small amounts on spleen and exudate granulocytes and a subpopulation of bone marrow cells, in moderate amounts on spleen and blood monocytes and expressed in much larger amounts on spleen histiocytes and peritoneal exudate macrophages. M1/70 antigen was absent from erythroid and lymphoid cells M1/70 antibody precipitated 2 polypeptides of 190,000 and 105,000 MW which were present in much greater amounts on peritoneal exudate macrophages than on spleen cells. The expression on phagocytes of 2 other antigens identified by monoclonal antibodies M1/69 and M1/9.3 was also examined. Monocytes and granulocytes expressed large amounts of M1/69 and low amounts of M1/70 antigen; in peritoneal exudate macrophages this pattern was dramatically reversed. M1/70 thus defines a differentiation antigen on mononuclear phagocytes and granulocytes, the expression of which is specifically increased during monocyte maturation. This antibody is the 1st to be described which recognizes a discrete molecule specific to phagocytes.