ROLE OF CHLOROPLAST FERREDOXIN IN ENERGY CONVERSION PROCESS OF PHOTOSYNTHESIS
ROLE OF CHLOROPLAST FERREDOXIN IN ENERGY CONVERSION PROCESS OF PHOTOSYNTHESIS
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DOI：
10.1073/pnas.49.4.567
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发表时间：
1963-01-01
影响因子：
11.1
通讯作者：
TSUJIMOTO, HY
中科院分区：
文献类型：
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作者：
TAGAWA, K;ARNON, DI;TSUJIMOTO, HY
方法：在所有实验中使用“破碎的”叶绿体28(P18或C18)。用于照明的白炽光通过Jena G1或G2滤光片过滤，该滤光片切断波长小于500m1a的光。形成的ATP用Hagihara和Lardy的方法估计。其他9个实验步骤与以前使用的相同。28结果和讨论-在我们实验室目前设想的机制中，10‘20循环和非循环的光磷酸化作用具有相同的初级光化学作用，在叶绿体内，引起从被激发的叶绿素分子到内源初级电子受体的电子流动。最近的研究结果表明，如果铁氧还蛋白是光化学反应后最早被分离出来的初级电子受体，那么铁氧还蛋白的光还原应该是循环或非循环光磷酸化的电子流动途径的共同特征。除了铁还蛋白外，这两种途径的电子受体也不同。在非环光磷酸化的情况下，来自铁还蛋白的电子将转移到TPN，
Methods.-" Broken" chloroplasts28 (P18 or C18) were used in all experiments. The incandescent light used for illumination was filtered through a Jena G1 or G2 filter, which cut off light of wavelength shorter than 500 m1A. The ATP formed was estimated by the method of Hagihara and Lardy.'9 Other experimental procedures were the same as thoseused previously. 28Results and Discussion.-In the mechanisms currently envisaged in our labora-tory, 10'20 cyclic and noncyclic photophosphorylation share the same primary photochemical act which gives rise, within the chloroplasts, to an electron flow from an excited chlorophyll molecule to an endogenous, primary electron acceptor. If, as recent results have shown, 6 ferredoxin is the earliest primary electron acceptor that can now be isolated following the photochemical act, then it follows that photo-reduction of ferredoxin should be a common feature of the electron flow pathways of either cyclic or noncyclic photophosphorylation. The two pathways would differ in the electron acceptors beyond ferredoxin. In the case of noncyclic photo-phosphorylation, the electrons from ferredoxin would be transferred to TPN,