LNAzymes: Incorporation of LNA-type monomers into DNAzymes markedly increases RNA cleavage
LNAzymes: Incorporation of LNA-type monomers into DNAzymes markedly increases RNA cleavage
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DOI：
10.1021/ja0276220
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发表时间：
2002-11-20
影响因子：
15
通讯作者：
Wengel, J
中科院分区：
文献类型：
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作者：
Vester, B;Lundberg, LB;Wengel, J
在“10-23”DNA酶的两个结合臂上分别掺入两个α-L-LNA/LNA核苷酸，并研究了它们对RNA的切割作用。与未修饰的DNAzyme相比，LNAzymes在单周转条件下显示出在小RNA底物（58 n RNA）中靶核苷酸处磷酸二酯骨架的显著改善的切割。LNAzymes显示有效的多重周转。使用LNA酶，还在高度结构化区域内的靶位点处实现了天然存在的核糖体RNA的有效切割。参考DNAzyme在切割核糖体RNA靶标时无效。
Incorporation of two α-L-LNA/LNA nucleotides into each of the two binding arms of a “10-23” DNAzyme has been accomplished and the RNA cleavage with these novel LNAzymes studied. In comparison with the unmodified DNAzyme, the LNAzymes show significantly improved cleavage of the phosphodiester backbone at the target nucleotide in a small RNA substrate (58n RNA) under single-turnover conditions. The LNAzymes show efficient multiple turnover. With the LNAzymes, efficient cleavage was accomplished also of a naturally occurring ribosomal RNA at a target site within a highly structured region. The reference DNAzyme was ineffective at cleaving the ribosomal RNA target.