Chromatin and RNAi factors protect the C-elegans germline against repetitive sequences
Chromatin and RNAi factors protect the C-elegans germline against repetitive sequences
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DOI：
10.1101/gad.332305
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发表时间：
2005-04-01
影响因子：
10.5
通讯作者：
Plasterk, RHA
中科院分区：
文献类型：
--
作者：
Robert, VJP;Sijen, T;Plasterk, RHA
保护基因组免受重复序列（如转座子、病毒和重复转基因）的侵袭涉及这些序列的强烈和选择性沉默。在重复转基因沉默期间，发生反式效应（“共抑制”），导致同源内源基因沉默。在这里，我们报告的RNA干扰（RNAi）屏幕进行目录所需的基因在秀丽隐杆线虫种系共抑制。我们发现了在染色质重塑中具有假定作用的因子和参与RNAi的因子。结合本研究中的分子数据，这些结果表明，在C。线虫重复序列通过RNAi和染色质因子触发转录基因沉默。
Protection of genomes against invasion by repetitive sequences, such as transposons, viruses, and repetitive transgenes, involves strong and selective silencing of these sequences. During silencing of repetitive transgenes, a trans effect ("cosuppression") occurs that results in silencing of cognate endogenous genes. Here we report RNA interference (RNAi) screens performed to catalog genes required for cosuppression in the Caenorhabditis elegans germline. We find factors with a putative role in chromatin remodeling and factors involved in RNAi. Together with molecular data also presented in this study, these results suggest that in C. elegans repetitive sequences trigger transcriptional gene silencing using RNAi and chromatin factors.