Arabidopsis thaliana FAR-RED ELONGATED HYPOCOTYLS3 (FHY3) and FAR-RED-IMPAIRED RESPONSE1 (FAR1) modulate starch synthesis in response to light and sugar
Arabidopsis thaliana FAR-RED ELONGATED HYPOCOTYLS3 (FHY3) and FAR-RED-IMPAIRED RESPONSE1 (FAR1) modulate starch synthesis in response to light and sugar
复制标题
拟南芥远红伸长下胚轴3 (FHY3) 和远红受损响应1 (FAR1) 调节淀粉合成以响应光和糖
DOI：
10.1111/nph.14300
复制
发表时间：
2017-03-01
期刊：
影响因子：
9.4
通讯作者：
Li, Gang
中科院分区：
文献类型：
--
作者：
Ma, Lin;Xue, Na;Li, Gang
在生物体中，每天的光/暗周期深刻地影响着细胞过程。在植物中，最佳的生长和发育以及对每日光暗周期的适应需要淀粉的合成和周转。为了探讨拟南芥光信号转导蛋白FHY 3和FAR 1在淀粉代谢中的作用，本研究以拟南芥光信号转导蛋白FHY 3和FAR 1为材料，研究了FHY 3和FAR 1中淀粉和水溶性多糖的含量以及淀粉颗粒的结构，FHY 3或FAR 1的破坏减少了fhy 3 -4、far 1 -2和fhy 3 -4 far 1 -2突变体植物中的淀粉积累并改变了淀粉颗粒结构。此外，分子和遗传学证据表明，编码淀粉脱支酶ISOAMYLASE 2（ISA 2）的基因是FHY 3和FAR 1的直接靶点，并在光诱导的淀粉合成中起作用。我们的数据建立了光信号转导和淀粉合成之间的第一个分子联系，暗示着光-信号蛋白FHY 3和FAR 1通过转录激活ISA 2影响淀粉合成和淀粉颗粒形成。
In living organisms, daily light/dark cycles profoundly affect cellular processes. In plants, optimal growth and development, and adaptation to daily light-dark cycles, require starch synthesis and turnover. However, the underlying molecular mechanisms coordinating daily starch metabolism remain poorly understood.To explore the roles of Arabidopsis thaliana light signal transduction proteins FAR-RED ELONGATED HYPOCOTYLS3 (FHY3) and FAR-RED-IMPAIRED RESPONSE1 (FAR1) in starch metabolism, the contents of starch and water-soluble polysaccharides, and the structure of starch granules were investigated in fhy3, far1 and fhy3 far1 mutant plants.Disruption of FHY3 or FAR1 reduced starch accumulation and altered starch granule structure in the fhy3-4, far1-2, and fhy3-4 far1-2 mutant plants. Furthermore, molecular and genetic evidence revealed that the gene encoding the starch-debranching enzyme ISOAMYLASE2 (ISA2) is a direct target of FHY3 and FAR1, and functions in light-induced starch synthesis.Our data establish the first molecular link between light signal transduction and starch synthesis, suggesting that the light-signaling proteins FHY3 and FAR1 influence starch synthesis and starch granule formation through transcriptional activation of ISA2.