Effect of oxide nanoparticles on the morphology and fluidity of phospholipid membranes and the role of hydrogen bonds
Effect of oxide nanoparticles on the morphology and fluidity of phospholipid membranes and the role of hydrogen bonds
复制标题
氧化物纳米颗粒对磷脂膜形貌、流动性的影响及氢键的作用
DOI：
10.1016/j.jes.2017.02.011
复制
发表时间：
2017
影响因子：
6.9
通讯作者：
Jiang Wei
中科院分区：
文献类型：
--
作者：
Wei Xiaoran;Yu Junchao;Ding Lei;Hu Jingtian;Jiang Wei
工程氧化物纳米颗粒(NPs)广泛应用于绝缘体、催化剂、涂料、化妆品、纺织和半导体等领域。它们附着在细胞膜上可能导致细胞毒性。本研究以大、小单层囊泡为研究对象，研究了Al_2O_3、Fe_2O_3、SiO_2、TiO_2和ZnO纳米粒子对膜完整性和流动性的影响。Al_2O_3和SiO_2纳米颗粒破坏了带相反电荷的膜，表明了静电吸引的重要作用。而Fe_2O_3、TiO_2和ZnO纳米粒子不会像Al_2O_3和SiO_2纳米粒子那样引起严重的膜破坏。用Laurdan荧光发射的广义极性(GP)值评价膜的流动性。SiO_2纳米粒子对带正负电膜均有诱导凝胶化作用。Al_2O_3和氧化锌纳米颗粒对带相反电荷的膜有凝胶作用，但对带相同电荷的膜没有明显的凝胶化作用。用傅立叶变换红外光谱(FT-IR)分析了NP暴露后磷脂分子结构的变化。FT-IR分析表明，纳米粒子与磷脂上的羰基/磷酸基团形成了氢键。在傅立叶变换红外光谱中，Al_2O_3和SiO_2纳米粒子的氢键作用最为明显。这与显微镜观察和荧光数据相一致，表明纳米氧化铝和二氧化硅纳米颗粒对膜的破坏和凝胶化更为严重。对膜损伤的研究为进一步了解纳米材料的细胞毒性和纳米粒应用的安全性提供了依据。
Engineered oxide nanoparticles (NPs) are widely applied in insulators, catalyzers, paints, cosmetic products, textiles and semiconductors. Their attachment on cell membrane may lead to cytotoxicity. The effects of Al2O3, Fe2O3, SiO2, TiO2and ZnO NPs on membrane integrity and fluidity were studied using giant or small unilamellar vesicles in this study. Al2O3and SiO2NPs disrupted the oppositely charged membrane, indicating the important role of electrostatic attraction. However, Fe2O3, TiO2and ZnO NPs did not cause serious membrane disruption as Al2O3and SiO2NPs. Membrane fluidity was evaluated by the generalized polarity (GP) values of Laurdan fluorescent emission. SiO2NPs induce the membrane gelation of both positively and negatively charged membrane. Al2O3and ZnO NPs induced the gelation of the oppositely charged membrane, but did not cause obvious membrane gelation to the like charged membrane. The phospholipid molecular structural changes after NP exposure were analyzed by Fourier transform infrared (FT-IR) spectroscopy. FT-IR spectra revealed the hydrogen bond formation between NPs and the carbonyl/phosphate groups of phospholipids. Al2O3and SiO2NPs showed strongest evidence of hydrogen bonding on their FT-IR spectra. It was consistent with the microscopic observation and fluorescent data that Al2O3and SiO2NPs caused more serious membrane disruption and gelation. This study on membrane damage provides further knowledge on the cytotoxicity of nanomaterials and the safety of NP application.