Function and molecular aspects of biomembrane transport: Edited by E Quagliariello, F Palmieri, S Papa, and M Klingenberg. Elsevier/North Holland Biomedical Press, Amsterdam and New York. 1979. $68.25. ISBN 0-444-80149-9
Function and molecular aspects of biomembrane transport: Edited by E Quagliariello, F Palmieri, S Papa, and M Klingenberg. Elsevier/North Holland Biomedical Press, Amsterdam and New York. 1979. $68.25. ISBN 0-444-80149-9
复制标题
生物膜运输的功能和分子方面：E Quagliariello、F Palmieri、S Papa 和 M Klingenberg 编辑。
DOI：
10.1016/0307-4412(81)90071-6
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
J. Kenny
中科院分区：
文献类型：
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作者：
J. Kenny
在分类学和进化中使用生物化学特征而不是形态学特征的优点之一是，这些特征相对不受环境诱导的变化的影响，因此人们对生物化学系统学越来越感兴趣。在这本书中，作者描述了比较生化研究中使用的各种技术，例如氨基酸测序、核酸分析、免疫学方法和凝胶电泳。这是最后提到的技术，这本书的大部分是专门的。作者给出了各种电泳技术的实际细节，并讨论了每种技术的可能性和局限性。这本书是针对大学生的，它决不是电泳的全面介绍。希望进一步探讨这一主题的人最好查阅附录中详细列出的补充参考资料。书包括关于种内变异和系统学的部分，种和属水平系统学，更高类别系统学，杂种和多倍体和一章处理生化和形态系统学之间的比较。在处理种内变异的部分中，作者讨论了电泳所揭示的酶多态性的生物学意义。这是一个核心问题的中性与选择假说的遗传变异的自然群体，这是一个遗憾的是，作者是不知道最近的文献有关这一争议。在进一步的章节中，作者用适当的例子描述了电泳在渔业管理中的应用，如鱼类种群的划界，杂种和多倍体的特征，以及阐明不同动植物群体的繁殖系统。毫无疑问，电泳对物种水平系统学的最重要贡献是在鉴定兄弟物种方面。在这里，作者引用了许多例子，从原生动物到哺乳动物，包括白色鱼复合体（白鲑属）-作者自己的研究兴趣。以上属水平的电泳是有限的使用在推断系统发育关系和更合适和精确的方法，如氨基酸测序，微量补体固定，DNA杂交，在这本书中描述。作者清楚而简明地论述了测量进化距离的各种方法，在关于电泳数据分析的最后一章中，他描述了一些用于构建树状图的方法。任何对进化和分类学感兴趣的人都应该读这本书。然而，价格可能排除了个人购买的那些它的目的，即先进的本科生和研究生。
One of the advantages of using biochemical as opposed to morphological characters in taxonomy and evolution is that such characters are relatively free of environmentally-induced changes-hence the growing interest in biochemical systematics. In this book the author describes the various techniques used in comparative biochemical studies, eg amino-acid sequencing, nucleic acid analysis, immunological methods, and gel electrophoresis. It is to the last mentioned technique that the greater part of the book is devoted. The author gives practical details of various electrophoretic techniques and discusses the possibilities and limitations of each. The book, which is aimed at the undergraduate student, is by no means a comprehensive account of electrophoresis. Those wishing to pursue this topic further would be well advised to consult the supplementary references detailed in the Appendix. The book includes sections on intraspecific variation and systematics, species-and genus-level systematics, higher category systematics, hybrids and polyploids and a chapter dealing with the comparisons between biochemical and morphological systematics. In the section dealing with intraspecific variation the author discusses the biological significance of enzyme polymorphism as revealed by electrophoresis. This is a question central to the neutralist versus selectionist hypotheses of genetic variability in natural populations and it is a pity that the author is unaware of much of the recent literature pertaining to this controversy. In further chapters the author describes, with suitable examples, how electrophoresis has been applied in fishery management, eg delimitation of fish stocks, in the characterization of hybrids and polyploids and in elucidating breeding systems in diverse groups of plants and animals. Undoubtedly, the most important contribution of electrophoresis to species-level systematics has been in the identification of sibling species. Here the author cites many examples ranging from protozoans to mammals and includes the white fish complex (genus Coregonus)-the author's own research interest. Above the genus level electrophoresis is of limited use in deducing phylogenetic relationships and more suitable and precise methods, eg amino-acid sequencing, microcomplement fixation, DNA hybridization, are described in this book. The author deals clearly and concisely with the various ways of measuring evolutionary distance and in the final chapter on the analysis of electrophoretic data he describes a few of the methods used in the construction of dendrograms. Anyone with an interest in evolution and taxonomy should read this book. The price however, probably precludes personal purchase by those for which it was intended namely the advanced undergraduate and postgraduate student.