Intratracheal administration of third-generation lentivirus vector encoding MPT51 from Mycobacterium tuberculosis induces specific CD8+ T-cell responses in the lung.
Intratracheal administration of third-generation lentivirus vector encoding MPT51 from Mycobacterium tuberculosis induces specific CD8+ T-cell responses in the lung.
复制标题
DOI：
10.1016/j.vaccine.2008.03.101
复制
发表时间：
2008-09
期刊：
影响因子：
5.5
通讯作者：
D. Hashimoto;T. Nagata;M. Uchijima;S. Seto;T. Suda;K. Chida;H. Miyoshi;Hirotoshi Nakamura;Y. Koide
中科院分区：
文献类型：
--
作者：
D. Hashimoto;T. Nagata;M. Uchijima;S. Seto;T. Suda;K. Chida;H. Miyoshi;Hirotoshi Nakamura;Y. Koide
本研究评估了改进的第三代慢病毒载体作为体内施用的结核病 T 细胞疫苗的潜力。气管内给予结核分枝杆菌MPT51慢病毒载体，给药后2周可在纵隔淋巴结中诱导MPT51特异性CD8+T细胞。疫苗接种可以在肺部产生 MPT51 特异性记忆 CD8+ T 细胞，但不能在淋巴结中产生。此外，MPT51慢病毒疫苗的单次气管内免疫显着减少了气管内芽孢杆菌攻击后肺部强毒力结核分枝杆菌的数量。这些发现表明，第三代慢病毒疫苗的气管内免疫是一种有前途的肺结核疫苗接种策略。
The present study evaluates the potential of improved third-generation lentivirus vector with respect to their use as an in vivo-administered T-cell vaccine against tuberculosis. Intratracheal administration of the lentivirus vector encoding MPT51 of Mycobacterium tuberculosis could induce MPT51-specific CD8+ T cells in the mediastinal lymph nodes 2 weeks after the administration. The vaccination could generate MPT51-specific memory CD8+ T cells in the lung, but not in the lymph nodes. Further, a single intratracheal immunization of MPT51 lentiviral vaccine decreased significantly the number of virulent M. tuberculosis in the lung after intratracheal challenge of the bacillus. These findings suggest that intratracheal immunization of the third-generation lentiviral vaccines is a promising vaccination strategy against pulmonary tuberculosis.