The effect of force on thermodynamics and kinetics: unfolding single RNA molecules.

The effect of force on thermodynamics and kinetics: unfolding single RNA molecules.
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力对热力学和动力学的影响:展开单个 RNA 分子。

DOI:
10.1042/bst0320757
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发表时间:
2004
影响因子:
3.9
通讯作者:
Li,PTX
Li,PTX
中科院分区:
生物学3区
文献类型:
--
作者:
TinocoJr,I;Collin,D;Li,PTX

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我们已经使用激光镊子在室温和生理型溶剂中展开单个RNA分子。展开RNA所需的力在10-20 pN的范围内,这些力可以由细胞酶产生。发现在添加烟酰胺后,HIV中TAR(反式激活响应)RNA解折叠的吉布斯自由能增加; TAR发夹稳定。去折叠速率降低,折叠速率增加。
We have used laser tweezers to unfold single RNA molecules at room temperature and in physiological-type solvents. The forces necessary to unfold the RNAs are over the range 10–20 pN, forces that can be generated by cellular enzymes. The Gibbs free energy for the unfolding of TAR (transactivation-responsive) RNA from HIV was found to be increased after the addition of argininamide; the TAR hairpin was stabilized. The rate of unfolding was decreased and the rate of folding was increased by argininamide.
DOI: 10.1016/s0076-6879(03)61009-8
发表时间: 2003
影响因子: --
作者:
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通讯作者: Steven B. Smith;Yujia Cui;C. Bustamante
DOI: 10.1073/pnas.0401799101
发表时间: 2004-05-11
影响因子: 11.1
作者:
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DOI: 10.1073/pnas.90.8.3680
发表时间: 1993-04-15
影响因子: 11.1
作者:
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通讯作者: WILLIAMSON, JR
DOI: 10.1006/jmbi.1995.0555
发表时间: 1995-10-20
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