HIGH CELL-DENSITY ALTERS THE RATIO OF TYPE-III TO TYPE-I COLLAGEN-SYNTHESIS BY FIBROBLASTS
HIGH CELL-DENSITY ALTERS THE RATIO OF TYPE-III TO TYPE-I COLLAGEN-SYNTHESIS BY FIBROBLASTS
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DOI：
10.1038/279442a0
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发表时间：
1979-01-01
期刊：
影响因子：
64.8
通讯作者：
MARTIN, GR
中科院分区：
文献类型：
--
作者：
ABE, S;STEINMANN, BU;MARTIN, GR
培养的人成纤维细胞同时合成I型和III型胶原1，其中I型占总数的70%-90%。在培养中，这些蛋白质的合成速度是恒定的，而且显然受到严格的控制。然而，这些胶原蛋白的比例在用增加的血清培养的细胞4以及从某些疾病患者获得的细胞中是不同的。来自Ehler-Danlos IV型综合征患者的细胞很少或根本不产生III型胶原5，6，而来自成骨不全患者的细胞产生更多的III型胶原(参考文献7，8)。我们发现，一些系统性硬化症(硬皮病)患者的细胞III型/I型比率降低。然而，正如之前报道的那样，这些细胞生长到的密度低于对照细胞9。我们在此报道，人和豚鼠皮肤的正常成纤维细胞在高细胞密度下按比例产生更多的III型胶原，可能是因为I型胶原的合成减少。
HUMAN fibroblasts in culture synthesise both type I and type III collagen1, with type I accounting for 70–90% of the total2. In culture, the rates at which these proteins are synthesised is constant and apparently rather rigidly controlled3. However, the proportions of these collagens differs in cells cultured with increased amounts of serum (increased type III/I)4as well as in cells obtained from patients with certain diseases. Cells from patients with the Ehlers-Danlos type IV syndrome make little or no type III collagen5,6, whereas cells from patients with osteogenesis imperfecta have an increased type III/I (refs 7, 8). We have found that cells from some patients with systemic sclerosis (scleroderma), have a reduced type III/I ratio. However, as previously reported, these cells grew to a lower density than control cells9. We report here that normal fibroblasts from human and guinea pig skin produce proportionally more type III collagen at high cell density, probably because of a reduction in the synthesis of type I collagen.