On-chip testing device for electrochemotherapeutic effects on human breast cells
On-chip testing device for electrochemotherapeutic effects on human breast cells
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DOI：
10.1007/s10544-008-9220-5
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发表时间：
2009-02-01
影响因子：
2.8
通讯作者：
Park, Je-Kyun
中科院分区：
文献类型：
--
作者：
Choi, Youn-Suk;Kim, Hong-Bae;Park, Je-Kyun
在保持电场强度分布相似性的前提下，将临床广泛使用的双针阵列电穿孔仪小型化为二维平面电极，研制了一种基于微细胞的电化学化疗（ECT）检测装置。该装置采用传统的二维细胞培养方法，在芯片上完成从细胞培养到电穿孔和最终基于细胞的检测的所有生物过程，利用单个装置中的6个电穿孔位点可以实现多次电化学检测。利用所建立的平台，用碘化丙啶评价电穿孔率，用不同电场强度和药物浓度的T47D人乳腺导管癌细胞株测定博来霉素化疗48 h后的细胞增殖。该微系统与传统的试管型电穿孔试验相比有几个优点，例如在芯片上进行多次测定，从细胞培养到最终测定的芯片上操作，并且具有与临床电穿孔仪相似的电场分布。随着电疗的临床试验的开展，这个新的平台有望在体外电痉挛基础研究、药物发现和临床电痉挛设备的开发中具有重要的应用价值。
A microfabricated cell-based testing device for electrochemotherapy (ECT) has been developed by miniaturizing the widely used clinical electroporator with a two-needle array into two-dimensional planar electrodes while keeping the similarity of the electric field strength distribution. In this device, all the biological processes from cell culture to electroporation and final cell-based assays were carried out on a chip using a conventional 2D cell culture method, and the multiple electrochemotherapeutic assays could be realized by exploiting the six electroporation sites in a single device. With the proposed platform, the electroporation rate was evaluated with propidium iodide and cell proliferation after 48 h of electrochemotherapy with bleomycin was determined with T47D human breast ductal carcinoma cell line in various electric field strengths and drug concentrations. This microsystem has several advantages over conventional cuvette type electroporation assay, such as multiple assays on a chip, on-chip based operation from cell culture to final assay, and having similar electric field distribution as that of the clinical electroporator. As the clinical trials of electrochemotherapy are being carried out, this new platform is expected to have valuable applications in basic in vitro ECT studies, drug discovery, and development of clinical ECT equipment.