Mutation of the key residue for extraribosomal function of ribosomal protein S19 cause increased grooming behaviors in mice.
Mutation of the key residue for extraribosomal function of ribosomal protein S19 cause increased grooming behaviors in mice.
复制标题
核糖体蛋白 S19 核糖体外功能的关键残基的突变导致小鼠的梳理行为增加。
DOI：
10.1016/j.neulet.2016.07.022
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发表时间：
2016
影响因子：
2.5
通讯作者：
Tetsuro Yamamoto
中科院分区：
文献类型：
--
作者：
Jun Chen;Taku Kaitsuka;Rika Fujino;Kimi Araki;Kazuhito Tomizawa;Tetsuro Yamamoto
核糖体蛋白S19（RP S19）具有作为RP S19单体的核糖体功能和作为交联的RP S19寡聚体的核糖体外功能，所述交联的RP S19寡聚体作为补体5a（C5 a）受体（CD 88）的配体起作用。我们已经产生了Gln 137 Glu-RP S19基因敲入（KI）小鼠，这表明具有减弱的核糖体外功能的RP S19。由于RP S19的核糖体外功能是否在脑功能中起作用尚不清楚，我们对这些小鼠进行了行为分析，并证明KI小鼠在旷场测试和高架十字迷宫测试中表现出增加的梳理行为，并在情境恐惧条件化测试中表现出增强的冻结行为。这些结果表明RP S19寡聚体参与一些焦虑样行为，特别是梳理行为。
Ribosomal protein S19 (RP S19) possesses ribosomal function as RP S19 monomer and extraribosomal function as cross-linked RP S19 oligomers which function as a ligand of the complement 5a (C5a) receptor (CD88). We have generated a Gln137Glu-RP S19 knock-in (KI) mouse, which is shown to possess the weakened extraribosomal function of RP S19. Because whether the extraribosomal function of RP S19 has a role in brain function had been unclear, we performed behavioral analysis on these mice and demonstrated that KI mice displayed an increased grooming behavior during open-field test and elevated plus maze test and an enhanced freezing behavior in contextual fear conditioning test. These results suggest an involvement of RP S19 oligomers in some anxiety-like behavior, especially grooming behavior.