Histological evaluation of platelet rich plasma and hydroxiapatite in apexogenesis: study on experimental animals
Histological evaluation of platelet rich plasma and hydroxiapatite in apexogenesis: study on experimental animals
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DOI：
10.2298/vsp0802128d
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发表时间：
2008-02-01
影响因子：
0.2
通讯作者：
Aleksic, Zoran
中科院分区：
文献类型：
--
作者：
Danilovic, Vesna;Petrovic, Vanja;Aleksic, Zoran
背景/目的。关于内源性生长因子在活髓治疗中的作用的数据很少，而且经常存在争议。本研究的目的是评价富血小板血浆(PRP)与羟基磷灰石(HAP)结合作为盖髓材料对牙根和牙周组织形成的影响。方法：研究方法。本研究涉及8只恒牙和牙根形成不完全的幼猴(Cercopithecus Aethiops)。牙髓切断术后，分别用氢氧化钙(对照组)、羟基磷灰石(实验1组)或羟基磷灰石与PRP联合覆盖(实验11组)。6个月后，处死动物，整块取出组织，按常规方法进行组织学分析。结果。组织学分析结果表明，修复过程以牙本质桥形成为特征，保持牙髓形态和功能的完整性，牙根及周围牙周组织完全形成。几乎所有样本的炎症反应评分均为轻度至中度，表明所用材料的生物相容性。结论。本研究中使用的材料作为盖盖剂使用方便，有助于保持牙髓组织的完整性，促进牙根和牙周组织的形成。根据组织学数据，可以认为羟基磷灰石与内源性生长因子结合，是本研究中使用的其他材料的最佳替代材料。
Background/Aim. There are very few data about the effects of endogenous growth factors in vital pulp therapy, and still they are often controversial. The aim of the study was to evaluate the effects of platelet rich plasma (PRP) in conjugation with hydroxyapatite (HAP), as pulp capping materials, to root and periodontium formation. Methods. Eight young monkeys (Cercopithecus Aethiops) with permanent dentition and incomplete root formation were involved in this study. After pulpotomy, the pulp lesion was capped with calcium hydroxide (control), hydroxyapatite (experimental group 1) or hydroxyapatite in conjugation with PRP (experimental group 11). Six months later, the animals were sacrificed, the tissue was removed en block, and prepared for the histological analysis in a routine way. Results. The results of the histological analysis revealed that healing process was characterised by dentin bridge formation, maintained morphological and functional integrity of dental pulp and complete formation of dental root and surrounding periodontium. The inflammatory reaction was scored as mild to moderate, in almost all the samples in all groups, suggesting the biocompatibility of the used materials. Conclusion. Materials used in this study are convenient as capping agents, contributing maintaining the integrity of the pulp tissue and facilitating root and periodontium formation. According to histological data it could be suggested that hydroxyapatite in conjugation with endogenous growth factors, represents superior alternative to other materials used in this study.