Active genes in budding yeast display enhanced in vivo accessibility to foreign DNA methylases: a novel in vivo probe for chromatin structure of yeast.
Active genes in budding yeast display enhanced in vivo accessibility to foreign DNA methylases: a novel in vivo probe for chromatin structure of yeast.
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芽殖酵母中的活性基因表现出增强的外源 DNA 甲基化酶的体内可及性：一种用于酵母染色质结构的新型体内探针。
DOI：
10.1101/gad.6.2.186
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发表时间：
1992
影响因子：
10.5
通讯作者：
Klar,AJ
中科院分区：
文献类型：
--
作者：
Singh,J;Klar,AJ
与高等真核生物不同，在基因表达和CpG位点甲基化之间通常观察到负相关，芽殖酵母酿酒酵母缺乏DNA甲基化。基因调控机制可以独立于酵母中的DNA甲基化发挥作用，并且已经发现表达修饰腺嘌呤和CpG残基的外源DNA甲基化酶的酵母菌株是可行的。我们已经使用这些菌株来确定基因的转录状态是否会影响其体内DNA甲基化水平。测试了几种基因，例如GAL 1、GAL-7和GAL-10、PHO 5、HMR α和HML α以及STE 2和STE 3。令人惊讶的是，我们发现所有基因在表达状态下的甲基化程度都是抑制状态的几倍。这一程序作为一种新的酵母染色质结构的体内探针，并可能为高等真核生物。
Unlike higher eukaryotes, where an inverse correlation has been generally observed between gene expression and methylation of CpG sites, the budding yeast Saccharomyces cerevisiae lacks DNA methylation. Gene regulatory mechanisms can function independently of DNA methylation in yeast, and yeast strains expressing foreign DNA methylases that modify adenine and CpG residues have been found to be viable. We have used such strains to determine whether the transcriptional status of genes can influence the level of their DNA methylation in vivo. Several genes were tested, for example, GAL1, -7, and -10, PHO5, HMRa and HML alpha, and STE2 and STE3. Surprisingly, we found that all the genes displayed severalfold more methylation in the expressed state as compared to the repressed state. This procedure serves as a novel in vivo probe for the chromatin structure of yeast and potentially for higher eukaryotes.