Effects of monovalent cations on folding kinetics of G-quadruplexes
Effects of monovalent cations on folding kinetics of G-quadruplexes
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单价阳离子对 G-四链体折叠动力学的影响
DOI：
10.1042/bsr20170771
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发表时间：
2017
影响因子：
4
通讯作者：
Dou SX
中科院分区：
文献类型：
--
作者：
You Jing;Li Hui;Lu Xi-Ming;Li Wei;Wang Peng-Ye;Dou Shuo-Xing;You Jing;Li Hui;Lu Xi-Ming;Li Wei;Wang Peng-Ye;Dou Shuo-Xing;You Jing;Li Hui;Lu Xi-Ming;Li Wei;Wang Peng-Ye;Dou Shuo-Xing;Xi Xu-Guang;Xi Xu-Guang;Li H;Dou SX;Li H;Dou SX;Li H;Dou SX
G-四链体是存在于人类端粒末端的一种特殊结构，其折叠动力学对其功能至关重要，如维持基因组稳定性和保护染色体末端。在本研究中，我们研究了G-四链体在不同单价阳离子环境中的折叠动力学，并确定了Na+和K+诱导的G-四链体折叠的详细动力学参数，以及其结构从篮式Na+形式到混合型K+形式的转变。更有趣的是，虽然Li+在以前的G-四链体折叠研究中经常被用作控制离子，假设没有影响，但我们发现Li+实际上可以以不同的方式显著影响Na+和K+诱导的G-四链体的折叠动力学，通过改变Na+诱导的G-四链体的折叠分数和大大增加K+诱导的G-四链体的折叠速率。本研究为进一步研究单价阳离子在G-四链体折叠中的作用提供了新的思路。
G-quadruplexes are special structures existing at the ends of human telomeres, the folding kinetics of which are essential for their functions, such as in the maintenance of genome stability and the protection of chromosome ends. In the present study, we investigated the folding kinetics of G-quadruplex in different monovalent cation environments and determined the detailed kinetic parameters for Na+- and K+-induced G-quadruplex folding, and for its structural transition from the basket-type Na+form to the hybrid-type K+form. More interestingly, although Li+was often used in previous studies of G-quadruplex folding as a control ion supposed to have no effect, we have found that Li+can actually influence the folding kinetics of both Na+- and K+-induced G-quadruplexes significantly and in different ways, by changing the folding fraction of Na+-induced G-quadruplexes and greatly increasing the folding rates of K+-induced G-quadruplexes. The present study may shed new light on the roles of monovalent cations in G-quadruplex folding and should be useful for further studies of the underlying folding mechanism.