Rapid pulsed-field gel electrophoresis method for group B streptococcus isolates
Rapid pulsed-field gel electrophoresis method for group B streptococcus isolates
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DOI：
10.1128/jcm.39.8.3006-3008.2001
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发表时间：
2001-08-01
影响因子：
9.4
通讯作者：
Ferrieri, P
中科院分区：
文献类型：
--
作者：
Benson, JA;Ferrieri, P
我们开发了一种快速脉冲场凝胶电泳（PFGE）方法，需要3天完成，比标准方法需要多达8天的改进。通过对对照组B链球菌分离物DNA条带大小的分析，验证了该方法的准确性和重复性。快速方法优于标准方法，提供更精确的分子大小和更高的图像质量的凝胶。快速PFGE的重复性证明了它的价值和继续使用。
We developed a rapid pulsed-field gel electrophoresis (PFGE) method that required 3 days to complete, an improvement over the standard method that required as many as 8 days. The accuracy and reproducibility of the rapid method were verified by analysis of DNA band sizes of our control group B streptococcus isolate. The rapid method was superior to the standard method, providing more precise molecular sizing and gels of higher image quality. The reproducibility of rapid PFGE substantiated its value and continued use.