Infiltration of CD40-Positive Tumor-Associated Macrophages Indicates a Favorable Prognosis in Colorectal Cancer Patients
Infiltration of CD40-Positive Tumor-Associated Macrophages Indicates a Favorable Prognosis in Colorectal Cancer Patients
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DOI：
10.5754/hge12372
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发表时间：
2013-01-01
影响因子：
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通讯作者：
Unno, Michiaki
中科院分区：
文献类型：
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作者：
Kinouchi, Makoto;Miura, Koh;Unno, Michiaki
背景/目的：近年来，肿瘤相关巨噬细胞(TAMs)在免疫中的作用已有不同的讨论。我们用流式细胞术对结直肠癌组织及其相应正常组织中TAMs中的一系列细胞表面抗原进行了研究，以寻找这些患者的预后指标。方法：对31例结直肠癌患者癌组织及相应正常组织中CD14+巨噬细胞CD80、CD86、HLA-DR、CD1a、CD40、CD83阳性细胞数进行单因素和多因素分析，找出影响患者总生存期的预后指标。结果：癌组织中CD80+、CD86+和HLADR+TAMs的数量均高于相应的正常组织。反之，癌组织中CD40+、CD83+巨噬细胞数明显少于正常组织。多因素分析显示，CD40+TAM数、淋巴结转移和远处转移是影响结直肠癌患者预后的独立因素。结论：CD40+在结直肠癌组织中的密集分布提示预后良好，提示CD40在结直肠癌的肿瘤免疫中起重要作用。
Background/Aims: Recently the role of tumor-associated macrophages (TAMs) on immunity has been variously discussed. We studied a series of cell surface antigens in TAMs in colorectal cancer tissues and their corresponding normal tissues using flow cytometry to find out prognostic indicators of these patients. Methodology: We assessed the numbers of CD14+ macrophages positive for each of the cell surface antigens (CD80, CD86, HLA-DR, CD1a, CD40 and CD83) in cancer tissues and corresponding normal tissues among 31 patients with colorectal cancer, and performed the univariate and multivariate analysis to find out prognostic indicators for overall survival among the patients. Results: The numbers of CD80+, CD86+ and HLADR+ TAMs in the cancer tissues were higher than those in corresponding normal tissues. Inversely CD40+ and CD83+ macrophages in cancer tissues were less than those in normal tissues. With the multivariate analysis, the number of CD40+ TAMs, as well as lymph node metastasis and distant metastasis, was shown to be an independent prognostic factor of colorectal cancer patients. Conclusions: The dense infiltration of CD40+ TAM in colorectal cancer tissues indicates a favorable prognosis, which suggests that CD40 plays an important role in the tumor immunity of colorectal cancer.