Overexpression of IbpB enhances production of soluble active Streptomyces olivaceovirdis XynB in Escherichia coli.
Overexpression of IbpB enhances production of soluble active Streptomyces olivaceovirdis XynB in Escherichia coli.
复制标题
IbpB 的过表达可增强大肠杆菌中可溶性活性链霉菌 Streptomyces olivaceovirdis XynB 的产生。
DOI：
10.1016/j.bbrc.2009.10.026
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发表时间：
2009
影响因子：
3.1
通讯作者：
Z. Dong
中科院分区：
文献类型：
--
作者：
Xiaoyun Su;Shan Zhang;Li Wang;Z. Dong
大肠杆菌小分子热休克蛋白IbpB在体外可通过Hsp 100-Hsp 70再激活系统促进包涵体蛋白解聚。因此，推测IbpB的过表达可能能够促进蛋白从包涵体中解聚，从而获得可溶性更高的重组蛋白。IbpB的过表达实际上提高了在E. coli BL21（DE3）。令人惊讶的是，XynB从包涵体的解聚没有加速。过表达的IbpB似乎保护了不正确或部分折叠的XynB免于聚集，并介导了随后的重折叠。这些结果显示了提高E.杆菌
The small heat shock protein IbpB of Escherichia coli can accelerate protein disaggregation from inclusion body by Hsp100–Hsp70 re-activation system in vitro. It was therefore hypothesized that overexpression of IbpB might be able to promote protein disaggregation from inclusion body, by which more soluble recombinant proteins would be obtained. The overexpression of IbpB actually enhanced production of more active soluble XynB of Streptomyces olivaceovirdis in E. coli BL21(DE3). Surprisingly, the disaggregation of XynB from inclusion body was not accelerated. It seemed that the overexpressed IbpB protected improperly or partially folded XynB from aggregation and mediated the subsequent refolding. These results show potential of improving production of active heterologous proteins in E. coli.