ASSEMBLY OF MITOCHONDRIAL-MEMBRANE SYSTEM .17. LOCALIZATION ON MITOCHONDRIAL-DNA OF MUTATIONS LEADING TO A LOSS OF RUTAMYCIN-SENSITIVE ADENOSINE-TRIPHOSPHATASE
ASSEMBLY OF MITOCHONDRIAL-MEMBRANE SYSTEM .17. LOCALIZATION ON MITOCHONDRIAL-DNA OF MUTATIONS LEADING TO A LOSS OF RUTAMYCIN-SENSITIVE ADENOSINE-TRIPHOSPHATASE
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DOI：
10.1111/j.1432-1033.1976.tb10769.x
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
TZAGOLOFF, A
中科院分区：
文献类型：
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作者：
FOURY, F;TZAGOLOFF, A
分离到4个酿酒酵母细胞质突变体，其线粒体中rutamycin敏感的atp酶活性降低，但细胞色素氧化酶和nadh细胞色素c还原酶活性显著。遗传学研究表明，这些突变彼此紧密相连，并被分配到一个新的位点PHO1上。突变显示与OLI2位点的重组频率较低，表明与该标记有联系。然而，它们与OLI1位点无关。通过.**GRAPHIC**恢复野生型二倍体也表明atp酶突变与OLI2位点的连锁关系。保留携带OLI2的线粒体DNA片段的克隆。基于三耐药菌株突变体杂交产生的重组体和对存在于.**GRAPHIC**中的耐药标记的分析。PHO1位点位于位于PAR1和OLI2之间的DNA片段中。
Four cytoplasmic mutants of Saccharomyces cerevisiae showing loss of mitochondrial rutamycin-sensitive ATPase activity but having significant cytochrome oxidase and NADH-cytochrome c reductase were isolated. Genetic studies indicate the mutations to be closely linked to each other and were assigned to a new locus, PHO1. The mutations show a low frequency of recombination with the OLI2 locus, suggesting a linkage to this marker. They are not, however, linked to the OLI1 locus. Linkage of the ATPase mutations to the OLI2 locus is also indicated by restoration of wild-type diploids by .**GRAPHIC**. clones that retain the segment of mitochondrial DNA carrying OLI2. Based on the recombinants issued from crosses of the mutants with a triple drug-resistant strain and an analysis of the resistance markers present in .**GRAPHIC**. clones that are effective in restoring a wild-type phenotype, the PHO1 locus was placed in the segment of DNA located between PAR1 and OLI2.