piggyBac transformation of the New World screwworm, Cochliomyia hominivorax, produces multiple distinct mutant strains
piggyBac transformation of the New World screwworm, Cochliomyia hominivorax, produces multiple distinct mutant strains
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DOI：
10.1111/j.1365-2915.2004.0473.x
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发表时间：
2004-03-01
影响因子：
1.9
通讯作者：
Skoda, SR
中科院分区：
文献类型：
--
作者：
Allen, ML;Handler, AM;Skoda, SR
昆虫不育技术（SIT）计划旨在通过将大规模饲养的不育昆虫释放到害虫出没的地区来根除害虫物种。SIT的第一个主要实施是新世界螺旋虫根除计划，该计划成功地从美国大陆，墨西哥和中美洲大部分地区消灭了新世界螺旋虫（NWS），Cochliomyia hominivorax（Coquerel）（双翅目：丽蝇科）。目前，电离辐射被用于绝育，但转基因昆虫技术可以取代这种方法，提供一种更安全、更划算的替代方法。遗传转化方法已在NWS中得到证实，并通过Southern印迹杂交、PCR和元件插入接头测序得到验证。一个致命的插入突变和增强子检测样的表型表达变异的介绍和讨论。除了支持根除工作，转化方法提供了潜在的手段，以确定基因和检查基因功能的NWS。
Sterile insect technique (SIT) programs are designed to eradicate pest species by releasing mass-reared, sterile insects into an infested area. The first major implementation of SIT was the New World Screwworm Eradication Program, which successfully eliminated the New World screwworm (NWS), Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), from the Continental US, Mexico and much of Central America. Ionizing radiation is currently used for sterilization, but transgenic insect techniques could replace this method, providing a safer, more cost-effective alternative. Genetic transformation methods have been demonstrated in NWS, and verified by Southern blot hybridization, PCR and sequencing of element insertion junctions. A lethal insertional mutation and enhancer detection-like phenotypic expression variations are presented and discussed. In addition to supporting the eradication efforts, transformation methods offer potential means to identify genes and examine gene function in NWS.