Temperature-dependent changes of cell shape during heterophyllous leaf formation in Ludwigia arcuata (Onagraceae)
Temperature-dependent changes of cell shape during heterophyllous leaf formation in Ludwigia arcuata (Onagraceae)
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DOI：
10.1007/s00425-008-0715-3
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发表时间：
2008-02
期刊：
影响因子：
4.3
通讯作者：
Masashi Sato;M. Tsutsumi;Akane Ohtsubo;K. Nishii;Asuka Kuwabara;T. Nagata
中科院分区：
文献类型：
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作者：
Masashi Sato;M. Tsutsumi;Akane Ohtsubo;K. Nishii;Asuka Kuwabara;T. Nagata
虽然在沉水叶中表皮细胞的伸长被认为是异叶水生植物的一个共同特征，但在Ludwigia spanataWalt中没有观察到这种伸长。（柳叶菜科）。在本研究中，我们发现降低培养温度可诱导L. arcuata.由于这种沉水叶也显示出减少的表皮细胞排列在叶横轴上，这些数据表明，异叶叶形成inL。温度传感器是部分温度敏感的，即，表皮细胞的伸长是温度敏感的，而表皮细胞数量的减少不显示这种温度敏感性。为了阐明引起这种温度敏感性的机制，我们研究了乙烯的影响，乙烯在28°C的相对较高的培养温度下诱导气生枝形成沉水型叶。在23°C下，乙烯诱导细胞伸长和叶横轴上表皮细胞数量的减少，而在28°C下未观察到细胞伸长。此外，在23°C下，淹水和乙烯处理都诱导了发育中叶片表皮细胞中皮层微管（MT）排列的变化。在28°C下不会引起MT排列的这种变化。参与乙烯温度敏感反应的因素可能是温度敏感型异叶叶形成的关键。arcuata.
Although elongation of epidermal cells in submerged leaves is thought to be a common feature of heterophyllous aquatic plants, such elongation has not been observed inLudwigia arcuataWalt. (Onagraceae). In this study we found that reduced culture temperature induced the elongation of epidermal cells of submerged leaves inL. arcuata. Since such submerged leaves also showed a reduction in the number of epidermal cells aligned across the leaf transverse axis, these data indicate that heterophyllous leaf formation inL. arcuatais partially temperature sensitive, i.e., the elongation of epidermal cells was temperature sensitive while the reduction in the number of epidermal cells did not show such temperature sensitivity. To clarify the mechanisms that cause such temperature sensitivity, we examined the effects of ethylene, which induced the formation of submerged-type leaves on aerial shoots at the relatively high culture-temperature of 28°C. At 23°C, ethylene induced both cell elongation and reduction in the number of epidermal cells across the leaf transverse axis, while cell elongation was not observed at 28°C. Moreover, both submergence and ethylene treatment induced a change in the arrangement of cortical microtubules (MTs) in epidermal cells of developing leaves at 23°C. Such changes in the arrangement of MTs was not induced at 28°C. Factors involved in the temperature-sensitive response to ethylene would be critical for temperature-sensitive heterophyllous leaf formation inL. arcuata.