Insulin stimulates testosterone biosynthesis by human thecal cells from women with polycystic ovary syndrome by activating its own receptor and using inositolglycan mediators as the signal transduction system.
Insulin stimulates testosterone biosynthesis by human thecal cells from women with polycystic ovary syndrome by activating its own receptor and using inositolglycan mediators as the signal transduction system.
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DOI：
10.1210/jcem.83.6.4886
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发表时间：
1998-06
期刊：
影响因子：
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通讯作者：
J. Nestler;D. Jakubowicz;Aída Falcón de Vargas;C. Brik;Nitza Quintero;Francisco Medina
中科院分区：
文献类型：
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作者：
J. Nestler;D. Jakubowicz;Aída Falcón de Vargas;C. Brik;Nitza Quintero;Francisco Medina
为确定胰岛素是否通过激活自身受体，以肌醇多糖为信号转导系统，刺激多囊卵巢综合征患者卵巢睾酮的产生，对多囊卵巢综合征患者的卵泡膜细胞进行了分离和培养。胰岛素和胰岛素样生长因子I刺激卵泡膜睾酮的生物合成。胰岛素受体的抗体阻断取消了胰岛素的刺激作用，而有效的抗体阻断胰岛素样生长因子I受体并不改变胰岛素对卵泡膜睾酮生物合成的刺激。一种含有多糖的肌醇(INS-2)增加了膜内睾酮的生物合成。用抗肌醇多聚糖抗体(A23939或α-IGP)预先孵育细胞可消除胰岛素的刺激作用，但不能消除hCG的刺激作用。这些发现表明，肌醇多糖是胰岛素刺激人膜内睾酮生物合成的信号转导系统。
To determine whether insulin stimulates human ovarian testosterone production in the polycystic ovary syndrome by activating its own receptor and using inositolglycan mediators as the signal transduction system, thecal cells from polycystic ovary syndrome women were isolated and cultured. Insulin and insulin-like growth factor I stimulated thecal testosterone biosynthesis. Antibody blockade of the insulin receptor abolished insulin's stimulatory action, whereas effective antibody blockade of the insulin-like growth factor I receptor did not alter insulin's stimulation of thecal testosterone biosynthesis. A chiro-inositol containing glycan (INS-2) increased thecal testosterone biosynthesis. Preincubation of cells with an antiinositolglycan antibody (A23939 or alpha IGP) abolished insulin's stimulatory effect, but not that of hCG. These findings suggest that inositolglycans serve as the signal transduction system for insulin's stimulation of human thecal testosterone biosynthesis.