Nonadditive expression of lipid metabolism pathway-related genes in intestine of hybrids of Nile tilapia females (Oreochromis niloticus) and blue tilapia males (Oreochromis aureus)
Nonadditive expression of lipid metabolism pathway-related genes in intestine of hybrids of Nile tilapia females (Oreochromis niloticus) and blue tilapia males (Oreochromis aureus)
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雌性尼罗罗非鱼 (Oreochromis niloticus) 和雄性蓝罗非鱼 (Oreochromis aureus) 杂交种肠道中脂质代谢途径相关基因的非加性表达
DOI：
10.1007/s11033-018-4490-3
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发表时间：
2019
影响因子：
2.8
通讯作者：
Zhong Huan
中科院分区：
文献类型：
--
作者：
Zhou Yi;Zhang Xiaojin;Xu Qian;Yan Jinpeng;Yu Fan;Xiao Jun;Guo Zhongbao;Luo Yongju;Zhong Huan
非加性表达有助于杂种优势。本研究对尼罗罗非鱼(Oreochromis niloticus)♀ × 蓝罗非鱼(Oreochromis aureus)♂杂交种肠道中12个脂质代谢途径相关基因的表达谱进行了研究。将杂交体的基因表达分配为非加性表达模式组和加性表达模式组，并与尼罗罗非鱼和蓝罗非鱼的表达模式进行比较。在杂交体的肠道中，apoA4B 以中等水平表达，但 apoB 和 MTP 分别属于 ELD-B 和 ELD-N 类别。 LPL 和 LRP1 在杂交体中表现出超调上调，但 LDLR 被归为 ELD-B 类别。对于脂肪酸摄取相关基因，只有 FABP11a 被归类为具有越级上调的非加性表达，而 CD36 和 FABP3 被归类为杂种肠道中的加性表达。三酰甘油代谢中的两个基因，即 FAS 和 DGAT2，在杂交体中表现出超度上调。本研究中分析的大多数基因显示出非加性表达（12 个基因中的 8 个），并且有 5 个基因显示出越界上调。这些结果表明，与亲本相比，杂种中脂质代谢受到刺激。这些基因在杂种中的过度表达可能与生长和脂质利用活力有关。
Nonadditive expression contributes to heterosis in hybrids. In this study, the expression profiles of twelve lipid metabolism pathway-related genes were investigated in the intestine of Nile tilapia (Oreochromis niloticus) ♀ × blue tilapia (Oreochromis aureus) ♂ hybrid. The expression of genes from the hybrid were assigned to nonadditive and additive expression pattern groups and compared with expression patterns from Nile tilapia and blue tilapia. In the intestine of the hybrid, apoA4B was expressed at intermediate levels, but apoB and MTP were assigned to ELD-B and ELD-N categories, respectively. The LPL and LRP1 showed transgressive up-regulation in the hybrid, but LDLR was assigned to the ELD-B category. For fatty acid uptake related genes, only FABP11a was categorized as nonadditive expression with transgressive up-regulation, while CD36 and FABP3 were categorized as additive expression in the intestine of the hybrid. Two genes in triacylglycerol metabolism, namely, FAS and DGAT2, showed transgressive up-regulation in the hybrid. Most of the genes analyzed in the present study showed nonadditive expression (8 in 12), and five genes showed transgressive up-regulation. These results indicated that the stimulation of lipid metabolism in the hybrid compared to that of its parents. The hyperactive expression of these genes in the hybrid may be associated with the growth and lipid usage vigor.