Interacting processes in protein coagulation.
Interacting processes in protein coagulation.
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蛋白质凝固中的相互作用过程。
DOI：
10.1002/(sici)1097-0134(19991001)37:1
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发表时间：
1999
期刊：
影响因子：
2.9
通讯作者：
Palma,MU
中科院分区：
文献类型：
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作者：
SanBiagio,PL;Martorana,V;Emanuele,A;Vaiana,SM;Manno,M;Bulone,D;Palma-Vittorelli,MB;Palma,MU
目前，人们对蛋白质的自结合和沉积有着浓厚的兴趣。这通常涉及整个蛋白质或片段的构象变化。即使在低浓度下也可能发生，它是系统性淀粉样变性、阿尔茨海默病和朊病毒病以及其他神经退行性病变等病理的原因。现成的蛋白质在低浓度下表现出与构象变化相关的自结合特性，提供了非常方便的模型系统，能够为这类问题提供见解。在这里，我们报告了牛血清白蛋白的实验，表明这种蛋白质的构象变化过程向凝血所需的中间形式同时发生，并与另外两个过程相互作用：溶液的介观解混和蛋白质交联。这种三种相互作用过程的途径即使在非常低的浓度下也允许凝血，并且最近在非肽性聚合物的情况下也观察到这种情况。因此，它可能是聚合物凝固/凝胶的一个相当普遍的特征。蛋白质1999;37: 116 - 120。©1999 Wiley‐Liss, Inc。
A strong interest is currently focused on protein self‐association and deposit. This usually involves conformational changes of the entire protein or of a fragment. It can occur even at low concentrations and is responsible for pathologies such as systemic amyloidosis, Alzheimer's and Prion diseases, and other neurodegenerative pathologies. Readily available proteins, exhibiting at low concentration self‐association properties related to conformational changes, offer very convenient model systems capable of providing insight into this class of problems. Here we report experiments on bovine serum albumin, showing that the process of conformational change of this protein towards an intermediate form required for coagulation occurs simultaneously and interacts with two more processes: mesoscopic demixing of the solution and protein cross‐linking. This pathway of three interacting processes allows coagulation even at very low concentrations, and it has been recently observed also in the case of a nonpeptidic polymer. It could therefore be a fairly common feature in polymer coagulation/gelation. Proteins 1999;37: 116–120. © 1999 Wiley‐Liss, Inc.