Proliferation and differentiation of human fetal brain neural stem cells in vitro
Proliferation and differentiation of human fetal brain neural stem cells in vitro
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DOI：
10.2147/jn.s148794
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发表时间：
2018-01-01
影响因子：
3.3
通讯作者：
Wu, Ping
中科院分区：
文献类型：
--
作者：
McGrath, Erica L.;Gao, Junling;Wu, Ping
原代人胎脑神经干细胞是一种独特的非转基因模型系统，用于研究人类神经发育的分子机制，模拟人类疾病，筛选药物或验证新的治疗方法。它们还可以用于细胞移植，以治疗各种神经疾病。该协议详细介绍了我们可以用来在培养中扩增hNSCs的方法，以及如何将它们区分为不同的神经元谱系和星形胶质细胞。
Primary human fetal brain neural stem cells (hNSCs) are a unique non-genetically modified model system to study molecular mechanisms underlying human neural development, to model human diseases, and to screen drugs or validate new treatments. They may also be used for cell transplantation to treat various neurological diseases. This protocol details our methods that can be used to expand hNSCs in culture as well as how to differentiate them into various neuronal lineages and astrocytes.