Effects of Nigella sativa seed polysaccharides on type 2 diabetic mice and gut microbiota
Effects of Nigella sativa seed polysaccharides on type 2 diabetic mice and gut microbiota
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DOI：
10.1016/j.ijbiomac.2020.05.042
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发表时间：
2020-09-15
影响因子：
8.2
通讯作者：
Kang, Wenyi
中科院分区：
文献类型：
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作者：
Dong, Jing;Liang, Qiongxin;Kang, Wenyi
以高脂饲料喂养的2型糖尿病小鼠为模型，研究黑种草子多糖（NSSP）对2型糖尿病小鼠及其肠道菌群的影响。测定小鼠空腹血糖（FBG）、生化指标、骨骼肌中细胞因子如肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-1 β（IL-1 β）和磷酸化AKT（p-AKT）蛋白、膜葡萄糖转运蛋白4（GLUT 4）的表达水平以及肠道菌群的变化。结果表明，NSSP高剂量组能显著降低空腹血糖（FBG）、糖化血清蛋白（GSP）、甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、丙二醛（MDA）、TNF-α、IL-6、IL-1 β水平，显著升高胰岛素（INS）、高密度脂蛋白胆固醇（HDL-C）、总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）以及p-AKT和GLUT 4的表达水平。此外，高剂量的NSSP显著增加了小鼠肠道中_Muribaculaceae_Unclassified和Bacteroides的丰度，这些丰度在链脲佐菌素（STZ）处理后被显著抑制。提示NSSP可通过调节PI 3 K/AKT信号通路改善糖尿病小鼠的异常状态，同时改变肠道菌群结构。(C)2020爱思唯尔B. V.保留所有权利。
Effect of Nigella sativa seed polysaccharides (NSSP) on type 2 diabetic mice and its gut microbiota was investigated on the type 2 diabetic mice model feed by high-fat diet. Fasting blood glucose (FBG), biochemical parameters, expression levels of cytokines such as tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), interleukin-1 beta (IL-1 beta), and phosphor-AKT (p-AKT) protein, membrane glucose transporter 4 (GLUT4) in skeletal muscles, as well as the change of gut microbiota profile in mice model were measured. Results showed that the high-dose NSSP could significantly lower the levels of FBG, glycosylated serum protein (GSP), triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), TNF-alpha, IL-6 and IL-1 beta, and significantly increased insulin (INS), high-density lipoprotein cholesterol (HDL\\C), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT) and the expression levels of p-AKT and GLUT4 in mice. Besides, the high-dose NSSP has significantly increased the abundance off_Muribaculaceae_Unclassified and Bacteroides, which were significantly suppressed in the mice gut after the treatment of streptozotocin (STZ). These results indicated that NSSP could improve the abnormal state of diabetic mice by regulating the PI3K/AKT signaling pathway with simultaneous changes of the gut microbiota profile. (C) 2020 Elsevier B.V. All rights reserved.