A novel fibroblast growth factor receptor-5 preferentially expressed in the pancreas
A novel fibroblast growth factor receptor-5 preferentially expressed in the pancreas
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DOI：
10.1016/s0167-4781(00)00282-7
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发表时间：
2001-03-19
期刊：
影响因子：
--
通讯作者：
Koh, GY
中科院分区：
文献类型：
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作者：
Kim, I;Moon, SO;Koh, GY
利用人胚胎 cDNA 上的聚合酶链式反应，我们分离出了编码一种新型 504 个氨基酸蛋白的 cDNA，称为成纤维细胞生长因子受体 (FGFR)-5，它与已知的 FGFR 高度同源。 FGFR5 的 NH2 末端部分包含一个假定的分泌信号序列、三个典型的免疫球蛋白样结构域、六个半胱氨酸和一个酸性盒，但没有 HAV 基序。 The COOH-terminal portion of FGFR5 contains one transmembrane domain but no intracellular kinase domain. Recombinant FGFR5 expressed in COS-7 cells is not secreted. but recombinant truncated FGFR5 lacking the predicted transmembrane domain is secreted. Acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF) do not bind to FGFR5. Among 23 adult human tissues, FGFR5 mRNA is preferentially expressed in the pancreas. These results suggest that FGFR5 may provide a binding site for some other fibroblast growth factors and may regulate some pancreatic function. (C) 2001 Elsevier Science B.V. All rights reserved.
Using the polymerase chain reaction on human embryonic cDNAs, we isolated a cDNA encoding a novel 504 amino acid protein, termed fibroblast growth factor receptor (FGFR)-5, which is highly homologous to known FGFRs. The NH2-terminal portion of FGFR5 contains a putative secretory signal sequence, three typical immunoglobulin-like domains, six cysteines, and an acidic box, but no HAV motif. The COOH-terminal portion of FGFR5 contains one transmembrane domain but no intracellular kinase domain. Recombinant FGFR5 expressed in COS-7 cells is not secreted. but recombinant truncated FGFR5 lacking the predicted transmembrane domain is secreted. Acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF) do not bind to FGFR5. Among 23 adult human tissues, FGFR5 mRNA is preferentially expressed in the pancreas. These results suggest that FGFR5 may provide a binding site for some other fibroblast growth factors and may regulate some pancreatic function. (C) 2001 Elsevier Science B.V. All rights reserved.