Hyperthermic effect on exponential and plateau ascites tumor cells in vitro dependent on environmental pH1.
Hyperthermic effect on exponential and plateau ascites tumor cells in vitro dependent on environmental pH1.
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体外对指数和平台期腹水肿瘤细胞的热效应依赖于环境 pH1。
DOI：
10.2307/3574602
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发表时间：
1977
影响因子：
3.4
通讯作者：
J. Overgaard
中科院分区：
文献类型：
--
作者：
P. Bichel;J. Overgaard
在正常氧分压下，在pH6.4和7.2的高温处理（42/sup 0/C）后，研究了从非同步指数生长和平台生长PNJ腹水肿瘤中获得的肿瘤细胞的集落形成。研究表明，在pH 7.2的平台细胞比指数细胞对热更敏感。当pH从7.2改变到6.4时，发现指数细胞的过热细胞杀伤显著增加。此外，在pH 6.4，然后在7.2，高原细胞对热更敏感，但细胞存活率的相对下降是相当少的指数细胞。在pH 6.4的平台细胞仍然稍微更敏感的热比类似的处理指数PNJ细胞。因此，中度高温对指数期和平台期生长的PNJ腹水肿瘤细胞产生不同影响的根本原因似乎是环境酸度不同，但不能排除平台期细胞本身热敏感性略有增加的可能性。
Colony formation of tumor cells obtained from asynchronous exponential and plateau growth PNJ ascites tumors were studied after hyperthermic treatment (42/sup 0/C) at pH 6.4 and 7.2 under normal oxygen tension. The study revealed that at pH 7.2 plateau cells were more sensitive to heat than exponential cells. A pronounced increase in hyperthermic cell killing of exponential cells was found when the pH was changed from 7.2 to 6.4. Also, plateau cells were more sensitive to heat at pH 6.4 then at 7.2 but the relative decrease in cell survival was considerably less than in exponential cells. At pH 6.4 the plateau cells were still slightly more sensitive to heat than similarly treated exponential PNJ cells. Thus, an essential cause of the different effect of moderate hyperthermia on cells from exponential and plateau growth PNJ ascites tumors seems to be the different environment acidity but the possibility of a slightly increased heat sensitivity of plateau phase cells per se cannot be excluded.