Role of ROS modified human DNA in the pathogenesis and etiology of cancer
Role of ROS modified human DNA in the pathogenesis and etiology of cancer
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DOI：
10.1016/s0304-3835(99)00112-3
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发表时间：
1999-07-19
期刊：
影响因子：
9.7
通讯作者：
Ali, A
中科院分区：
文献类型：
--
作者：
Abdi, S;Ali, A
采用紫外光谱法、熔融温度法、S-1核酸酶消化率法和羟基磷灰石柱层析法检测了过氧化氢辐照产生的羟基自由基对人胎盘DNA的影响。免疫学数据表明，活性氧（ROS）修饰的人DNA诱导高效价抗体。在ELISA中，不同癌症患者的血清抗体对ros -人DNA的识别率高于天然DNA。从癌症血清中分离的IgG与ros -人DNA之间形成的免疫复合物的迁移迟缓，为抗原-抗体相互作用提供了令人信服的证据。采用抗ros -人DNA IgG检测肿瘤患者DNA氧化损伤。来自癌症患者的DNA被发现能抑制40% - 57%的抗ros -人DNA IgG活性。这些结合结果表明在癌症患者的基因组中存在氧化损伤。1999爱思唯尔科学爱尔兰有限公司版权所有。
The effect of hydroxyl radical, generated by ultraviolet (UV) irradiation of hydrogen peroxide, on human placental DNA was monitored by UV spectroscopy, melting temperature studies, S-1 nuclease digestibility and hydroxyapatite column chromatography. Immunological data indicated that reactive oxygen species (ROS) modified human DNA induced high titer antibodies. In ELISA, serum antibodies from various cancer patients showed a higher recognition of ROS-human DNA as compared to native DNA. Retarded mobility of the immune complex formed between IgG, isolated from cancer sera, and ROS-human DNA provided convincing evidence for antigen-antibody interaction. Oxidative lesions in DNA of cancer patients were probed using anti-ROS-human DNA IgG. DNA from cancer patients were found to inhibit anti-ROS-human DNA IgG activity in the range of 40% to 57%. These binding results indicate the presence of oxidative lesions in the cancer patient's genome. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.