In situ embedding and vertical sectioning for electron microscopy of tissue cultures grown on plastic petri dishes.
In situ embedding and vertical sectioning for electron microscopy of tissue cultures grown on plastic petri dishes.
复制标题
对塑料培养皿上生长的组织培养物进行电子显微镜的原位包埋和垂直切片。
作者：
B. K. Nelson;B. A. Flaxman
Cell cultures, grown on Falcon polystyrene Petri dishes, can be open-face embedded in situ with Araldite and then vertically sectioned for electron microscopy without first separating the embedding resin from the culture dish. The polystyrene dish has cutting properties sufficiently similar to Araldite so that the different plastics adhere firmly to each other during sectioning. Both 1–1.5 μ and 60–100 nm sections are easily obtained. This technique permits ultrastructural studies of cells cultured in vitro while keeping intact the cell-substratum interface.
