Proline can be utilized as an energy substrate during flight of Aedes aegypti females
Proline can be utilized as an energy substrate during flight of Aedes aegypti females
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DOI：
10.1016/s0022-1910(03)00031-3
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发表时间：
2003-06-01
影响因子：
2.2
通讯作者：
Wells, MA
中科院分区：
文献类型：
--
作者：
Scaraffia, PY;Wells, MA
为了确定脯氨酸是否可以在埃及伊蚊飞行过程中用作燃料，在飞行后 0、30 和 60 分钟，使用糖喂养的雄性和雌性以及血粉喂养的雌性监测脯氨酸、丙氨酸和谷氨酰胺浓度。在糖喂养和血粉喂养的雌性中，飞行导致血淋巴和胸部脯氨酸显着减少，谷氨酰胺浓度显着增加。仅在飞行期间吸血后观察到血淋巴中的丙氨酸浓度显着增加。飞行后，雄性血淋巴和胸部的脯氨酸丙氨酸和谷氨酰胺水平没有显着变化。此外，还测定了两性头胸部和胸部匀浆以及雌性脂肪体和中肠中与氨基酸代谢相关的酶的活性。在两性中，胸部所研究的所有酶的活性均显着高于头胸部。胸部参与脯氨酸氧化的酶水平高于脂肪体和中肠。这些结果表明脯氨酸可以用作伊蚊飞行肌的能量底物。埃及伊蚊雌性。然而，在其他使用脯氨酸为飞行提供燃料的昆虫中，尚未报道飞行后在血淋巴和胸部观察到谷氨酰胺水平升高的情况，这可能表明蚊子中存在一种在飞行肌肉和脂肪体之间穿梭氨的额外机制。 (C) 2003 Elsevier Science Ltd. 保留所有权利。
In order to determine whether proline can be utilized as fuel during flight of Aedes aegypti, proline, alanine, and glutamine concentrations were monitored at 0, 30 and 60 min after flight using sugar-fed males and females, and blood meal-fed females. In sugar-fed and blood meal-fed females, flight lead to a significant decrease in proline and a significant increase in glutamine concentration in both hemolymph and thorax. Only during flight after a blood meal was a significant increase in the alanine concentration observed in hemolymph. After flight, the proline alanine and glutamine levels in the hemolymph and thorax from males did not change significantly. In addition, activities of enzymes related to amino acid metabolism were assayed in homogenates of cephalothorax and thorax from both sexes, and in fat body and midgut from females. In both sexes, the activities of all the enzymes studied were significantly higher in thorax than in cephalothorax. The levels of the enzymes involved in proline oxidation were higher in thorax than in fat body and midgut. These results suggest that proline can be used as an energy substrate for flight muscle of Ae. aegypti females. However, the elevation in glutamine levels observed in hemolymph and thorax after flight has not been reported in other insects that fuel flight using proline and may suggest an additional mechanism for shuttling ammonia between flight muscle and fat body is present in mosquitoes. (C) 2003 Elsevier Science Ltd. All rights reserved.