Induction of platelet formation from megakaryocytoid cells by nitric oxide
Induction of platelet formation from megakaryocytoid cells by nitric oxide
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DOI：
10.1073/pnas.241427398
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发表时间：
2001-12-04
影响因子：
11.1
通讯作者：
Loscalzo, J
中科院分区：
文献类型：
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作者：
Battinelli, E;Willoughby, SR;Loscalzo, J
尽管调控巨核细胞生成的生长因子众所周知，但成熟巨核细胞形成血小板的分子决定因素仍然知之甚少。巨核细胞的形态变化与血小板的形成和衰老的巨核细胞的去除有关，提示细胞的凋亡过程。在此之前，我们已经证实一氧化氮(NO)可以诱导巨核细胞系的凋亡。为了确定NO诱导的细胞凋亡与血小板生成之间是否存在关联，我们将细胞暴露于含或不含促血小板生成素的S-亚硝基谷胱甘肽(GSNO)中，并用流式细胞仪和电子显微镜检测了血小板大小的颗粒形成。单独用TPO处理的MEG-01细胞产生的血小板大小的颗粒很少(
Although the growth factors that regulate megakaryocytopoiesis are well known, the molecular determinants of platelet formation from mature megakaryocytes remain poorly understood. Morphological changes in megakaryocytes associated with platelet formation and removal of senescent megakaryocytes are suggestive of an apoptotic process. Previously, we have established that nitric oxide (NO) can induce apoptosis in megakaryocytoid cell lines. To determine whether there is an association between NO-induced apoptosis and platelet production, we exposed Meg-01 cells to S-nitrosoglutathione (GSNO) with or without thrombopoeitin (TPO) pretreatment and used flow cytometry and electron microscopy to assess platelet-sized particle formation. Meg-01 cells treated with TPO alone produced few platelet-sized particles (