Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of (1)H-(1)H spin-spin coupling constants in proteins. 1983.
Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of (1)H-(1)H spin-spin coupling constants in proteins. 1983.
复制标题
应用相敏二维相关光谱 (COSY) 测量蛋白质中的 (1)H-(1)H 自旋-自旋耦合常数。
DOI：
10.1016/j.bbrc.2012.08.018
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发表时间：
2012
影响因子：
3.1
通讯作者：
K. Wüthrich
中科院分区：
文献类型：
--
作者：
D. Marion;K. Wüthrich
二维相关谱（COSY）被用于测量蛋白质1H NMR谱中质子-质子自旋-自旋耦合常数。沿频率轴沿着ω 2的高数字分辨率是通过将载波频率置于频谱中心、在两个维度上使用正交检波并以相敏模式呈现频谱来实现的。与其他研究自旋-自旋耦合常数的技术相比，COSY提供了大大提高的光谱分辨率。这通过使用小球状蛋白BUSI IIA（公牛精液抑制剂IIA）的H2O溶液的实验来说明。
Two-dimensional correlated spectroscopy (COSY) is used for measurements of proton-proton spin-spin coupling constants in protein1H NMR spectra. High digital resolution along the frequency axis ω2is achieved by placing the carrier frequency in the center of the spectrum, using quadrature detection in both dimensions and presenting the spectrum in the phase sensitive mode. Compared to other techniques for studies of spin-spin coupling constants, COSY provides greatly improved spectral resolution. This is illustrated by experiments with H2O solutions of the small globular protein BUSI IIA (bull seminal inhibitor IIA).