Ecklonia stolonifera Extract Suppresses Lipid Accumulation by Promoting Lipolysis and Adipose Browning in High-Fat Diet-Induced Obese Male Mice
Ecklonia stolonifera Extract Suppresses Lipid Accumulation by Promoting Lipolysis and Adipose Browning in High-Fat Diet-Induced Obese Male Mice
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DOI：
10.3390/cells9040871
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发表时间：
2020-04-01
期刊：
影响因子：
6
通讯作者：
Lee, Boo-Yong
中科院分区：
文献类型：
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作者：
Jin, Heegu;Lee, Kippeum;Lee, Boo-Yong
肥胖是由于能量不平衡而产生的，表现为白色脂肪组织 (WAT) 中储存了过量的甘油三酯 (TG)。最近的研究已经确定，可食用的天然材料可以减少WAT中的脂质积累并促进褐变。我们的目的是确定匍匐昆布提取物 (ESE) 是否会通过上调脂肪分解和褐变来增加高脂饮食 (HFD) 诱导的肥胖小鼠和 3T3-L1 细胞的能量消耗。 ESE 是一种可食用的棕色海洋藻类，属于海带科，含有二酚（一种叶皮单宁）。我们报告说，ESE 通过调节参与脂肪生成和脂肪生成的蛋白质的表达来抑制体重增加。此外，ESE 激活蛋白激酶 A (PKA) 并增加脂肪分解酶的表达，包括脂肪甘油三酯脂肪酶 (ATGL)、磷酸化激素敏感脂肪酶 (p-HSL) 和单酰甘油脂肪酶 (MGL) 以及产热基因，例如肉碱棕榈酰转移酶 1 (CPT1)、含 PR 结构域 16 (PRDM16) 和解偶联蛋白1（UCP1）。这些发现表明，ESE 可能是预防肥胖和肥胖相关代谢疾病的一种有前途的自然方法。
Obesity develops due to an energy imbalance and manifests as the storage of excess triglyceride (TG) in white adipose tissue (WAT). Recent studies have determined that edible natural materials can reduce lipid accumulation and promote browning in WAT. We aimed to determine whether Ecklonia stolonifera extract (ESE) would increase the energy expenditure in high-fat diet (HFD)-induced obese mice and 3T3-L1 cells by upregulating lipolysis and browning. ESE is an edible brown marine alga that belongs to the family Laminariaceae and contains dieckol, a phlorotannin. We report that ESE inhibits body mass gain by regulating the expression of proteins involved in adipogenesis and lipogenesis. In addition, ESE activates protein kinase A (PKA) and increases the expression of lipolytic enzymes including adipose triglyceride lipase (ATGL), phosphorylated hormone-sensitive lipase (p-HSL), and monoacylglycerol lipase (MGL) and also thermogenic genes, such as carnitine palmitoyltransferase 1 (CPT1), PR domain-containing 16 (PRDM16), and uncoupling protein 1 (UCP1). These findings indicate that ESE may represent a promising natural means of preventing obesity and obesity-related metabolic diseases.