COI barcoding of true bugs (Insecta, Heteroptera)
COI barcoding of true bugs (Insecta, Heteroptera)
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DOI：
10.1111/j.1755-0998.2010.02945.x
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发表时间：
2011-03-01
影响因子：
7.7
通讯作者：
Lee, Seunghwan
中科院分区：
文献类型：
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作者：
Jung, Sunghoon;Duwal, Ram K.;Lee, Seunghwan
最近的一些研究提出，细胞色素c氧化酶I （COI）线粒体基因的部分DNA序列可能作为识别和区分动物物种的DNA条形码，如鸟类、鱼类和昆虫。在这项研究中，我们测试了COI条形码识别来自韩国及其邻近地区（日本、中国东北和俄罗斯远东）的139种真虫的有效性。近缘种间的平均遗传距离约为种内遗传距离的16倍。DNA条形码鉴定出一种可能的真虫新种，并揭示了相同的或最近分化的物种，这些物种在形态特征上有明显的区别。因此，我们的研究结果表明，COI条形码可以揭示新的隐藏的真虫物种，并能够为真虫的准确鉴定做出贡献。
Several recent studies have proposed that partial DNA sequences of the cytochrome c oxidase I (COI) mitochondrial gene might serve as DNA barcodes for identifying and differentiating between animal species, such as birds, fish and insects. In this study, we tested the effectiveness of a COI barcode to identify true bugs from 139 species collected from Korea and adjacent regions (Japan, Northeastern China and Fareast Russia). All the species had a unique COI barcode sequence except for the genus Apolygus (Miridae), and the average interspecific genetic distance between closely related species was about 16 times higher than the average intraspecific genetic distance. DNA barcoding identified one probable new species of true bug and revealed identical or very recently divergent species that were clearly distinguished by morphological characteristics. Therefore, our results suggest that COI barcodes can reveal new cryptic true bug species and are able to contribute for the exact identification of the true bugs.