Maternal nitric oxide homeostasis impacts female gametophyte development under optimal and stress conditions
Maternal nitric oxide homeostasis impacts female gametophyte development under optimal and stress conditions
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母体一氧化氮稳态影响最佳和应激条件下雌配子体的发育
DOI：
10.1093/plcell/koae043
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Vierling, Elizabeth
中科院分区：
文献类型：
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作者：
Wang, Junzhe;Guo, Xiaolong;Chen, Yijin;Liu, Tianxiang;Zhu, Jianchu;Xu, Shengbao;Vierling, Elizabeth
在不利的环境中，植物中可受精的雌配子体（FGs）的数量减少，导致剩余后代的存活率增加。母体植物如何感知内部生长线索和外部胁迫条件，以改变FG的发展仍然在很大程度上未知。我们报告说，稳态的压力信号分子一氧化氮（NO）起着关键作用，在控制FG发展的最佳和压力条件下。NO稳态受S-亚硝基谷胱甘肽还原酶（GSNOR）的精确调节。在受精前，GSNOR蛋白专门积累在孢子体组织中，并间接控制拟南芥（Arabidopsis thaliana）的FG发育。在GSNOR无效突变体中，退化的孢子体珠心中积累了NO物质，生长素流出到发育中的FG中受到限制，这抑制了FG的发育，导致育性降低。重要的是，在母体而不是配子体组织中抑制GSNOR表达，或增加生长素外排底物显著增加了正常FGs和生育力的比例。此外，GSNO过量表达或添加生长素外排底物提高了干旱和盐胁迫下的育性。这些数据表明，NO稳态是至关重要的生长素运输和FG发展的母亲控制，这反过来又决定种子产量。了解这方面的生育控制可能有助于调解不利条件下的产量损失。
In adverse environments, the number of fertilizable female gametophytes (FGs) in plants is reduced, leading to increased survival of the remaining offspring. How the maternal plant perceives internal growth cues and external stress conditions to alter FG development remains largely unknown. We report that homeostasis of the stress signaling molecule nitric oxide (NO) plays a key role in controlling FG development under both optimal and stress conditions. NO homeostasis is precisely regulated byS-nitrosoglutathione reductase (GSNOR). Prior to fertilization, GSNOR protein is exclusively accumulated in sporophytic tissues and indirectly controls FG development in Arabidopsis (Arabidopsis thaliana). In GSNOR null mutants, NO species accumulated in the degenerating sporophytic nucellus, and auxin efflux into the developing FG was restricted, which inhibited FG development, resulting in reduced fertility. Importantly, restoringGSNORexpression in maternal, but not gametophytic tissues, or increasing auxin efflux substrate significantly increased the proportion of normal FGs and fertility. Furthermore,GSNORoverexpression or added auxin efflux substrate increased fertility under drought and salt stress. These data indicate that NO homeostasis is critical to normal auxin transport and maternal control of FG development, which in turn determine seed yield. Understanding this aspect of fertility control could contribute to mediating yield loss under adverse conditions.