Aptamer Conjugated Gold Nanostar-Based Distance-Dependent Nanoparticle Surface Energy Transfer Spectroscopy for Ultrasensitive Detection and Inactivation of Corona Virus
Aptamer Conjugated Gold Nanostar-Based Distance-Dependent Nanoparticle Surface Energy Transfer Spectroscopy for Ultrasensitive Detection and Inactivation of Corona Virus
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DOI：
10.1021/acs.jpclett.0c03570
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发表时间：
2021-02-25
影响因子：
5.7
通讯作者：
Ray, Paresh Chandra
中科院分区：
文献类型：
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作者：
Pramanik, Avijit;Gao, Ye;Ray, Paresh Chandra
持续爆发的冠状病毒感染已造成200多万人死亡。本研究证明，基于罗丹明6G （Rh-6G）染料共轭DNA配体附着金纳米星（GNS）的距离依赖纳米粒子表面能转移（NSET）光谱技术具有在10分钟内快速诊断特异性SARS-CoV-2刺突重组抗原或SARS-CoV-2刺突蛋白假型杆状病毒的能力。由于rh - 6g附着的单支架DNA适体包裹了GNS，因此由于NSET过程，99%的染料荧光被猝灭。在刺突抗原或病毒存在的情况下，由于适体与刺突蛋白结合，荧光信号持续存在。具体来说，NSET试验的检测限已确定为抗原130 fg/mL和病毒8颗粒/mL。最后，我们证明了DNA适配体附着的GNSs可以通过阻断血管紧张素转换酶2 （ACE2）受体的结合能力和破坏病毒的脂质膜来阻止病毒感染。
The ongoing outbreak of the coronavirus infection has killed more than 2 million people. Herein, we demonstrate that Rhodamine 6G (Rh-6G) dye conjugated DNA aptamer-attached gold nanostar (GNS)-based distance-dependent nanoparticle surface energy transfer (NSET) spectroscopy has the capability of rapid diagnosis of specific SARS-CoV-2 spike recombinant antigen or SARS-CoV-2 spike protein pseudotyped baculovirus within 10 min. Because Rh-6G-attached single-stand DNA aptamer wrapped the GNS, 99% dye fluorescence was quenched because of the NSET process. In the presence of spike antigen or virus, the fluorescence signal persists because of the aptamer-spike protein binding. Specifically, the limit of detection for the NSET assay has been determined to be 130 fg/mL for antigen and 8 particles/mL for virus. Finally, we have demonstrated that DNA aptamer-attached GNSs can stop virus infection by blocking the angiotensin-converting enzyme 2 (ACE2) receptor binding capability and destroying the lipid membrane of the virus.