Point of Care Tuberculosis Sero-Diagnosis Kit for Wild Animals: Combination of Proteins for Improving the Diagnostic Sensitivity and Specificity.
Point of Care Tuberculosis Sero-Diagnosis Kit for Wild Animals: Combination of Proteins for Improving the Diagnostic Sensitivity and Specificity.
复制标题
野生动物床旁结核病血清诊断试剂盒：蛋白质组合可提高诊断敏感性和特异性。
DOI：
10.1007/s12088-017-0688-7
复制
发表时间：
2018
影响因子：
3
通讯作者：
Veerasami M
中科院分区：
文献类型：
--
作者：
Veerasami M
结核病是全球家畜以及圈养和自由放养野生动物的一个重大问题。快速护理点（POC）血清学试剂盒非常适合野生动物结核病的诊断。然而，野生动物总是暴露于环境中的非致病性分枝杆菌物种与交叉反应抗体的发展。本研究利用致病性分枝杆菌特异性重组抗原和致病性和非致病性分枝杆菌的纯化蛋白衍生物，开发了POC TB诊断试剂盒。为了对TB抗体检测试剂盒进行基准测试，特别是在由于缺乏来自确认未感染动物的样品而无法在野生动物中确定的特异性方面，我们首先测试了来自100 M的充分表征的血清。100头未感染的牛。对74份懒熊、9份大象、14份鹿科动物、21份猫科动物、2份南非布法罗、1份野熊和1份野犬血清进行了检测，牛血清的敏感性为81%，特异性为90%。当使用已知TB阳性的懒熊血清样品测试试剂盒时，试剂盒的诊断灵敏度为94%。使用快速POC TB诊断试剂盒，47.4%的接触树懒熊转为血清阳性。用该试剂盒检测其他野生动物血清样品，阳性率为25%。开发了具有蛋白质组合的床旁结核血清诊断试剂盒，并使用野生动物血清样品对试剂盒进行了验证。
Tuberculosis is a significant problem globally for domestic animals as well as captive and free ranging wild life. Rapid point of care (POC) serology kits are well suited for the diagnosis of TB in wild animals. However, wild animals are invariably exposed to environmental non-pathogenic mycobacterium species with the development of cross reacting antibodies. In the present study, POC TB diagnosis kit was developed using a combination of pathogenicMycobacteriaspecific recombinant antigens and purified protein derivatives of pathogenic and non-pathogenicMycobacteria. To benchmark the TB antibody detection kit, particularly in respect to specificity which could not be determined in wildlife due to the lack of samples from confirmed uninfected animals, we first tested well-characterized sera from 100M. bovisinfected and 100 uninfected cattle. Then we investigated the kit’s performance using sera samples from wildlife, namely Sloth Bears (n = 74), Elephants (n = 9), Cervidae (n = 14), Felidae (n = 21), Cape buffalo (n = 2), Wild bear (n = 1) and Wild dog (n = 1).In cattle, a sensitivity of 81% and a specificity of 90% were obtained. The diagnostic sensitivity of the kit was 94% when the kit was tested using known TB positive sloth bear sera samples. 47.4% of the in-contact sloth bears turned seropositive using the rapid POC TB diagnostic kit. Seropositivity in other wild animals was 25% when the sera samples were tested using the kit. A point of care TB sero-diagnostic kit with the combination of proteins was developed and the kit was validated using the sera samples of wild animals.