The sequential seeding of epithelial and mesenchymal cells for tissue-engineered tooth regeneration
The sequential seeding of epithelial and mesenchymal cells for tissue-engineered tooth regeneration
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DOI：
10.1016/j.biomaterials.2006.09.039
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发表时间：
2007-02-01
期刊：
影响因子：
14
通讯作者：
Ueda, Minoru
中科院分区：
文献类型：
--
作者：
Honda, Masaki J.;Tsuchiya, Shuhei;Ueda, Minoru
通过将分离的出生后牙源性细胞接种到支架上并将其植入体内，牙齿再生正在取得进展，但牙齿形态仍然难以控制。在这项研究中，我们的目的是促进牙齿再生，使用一种新的技术，依次种子上皮细胞和间充质细胞，使他们在支架形成适当的相互作用。采用酶法分离猪第三磨牙牙上皮和间充质，并将其分离成单细胞。将间充质细胞接种到支架的表面上，然后将上皮细胞铺在上面，使得两种细胞类型直接接触。在体外评估细胞支架构建体，并将其植入免疫功能低下的大鼠中进行体内分析。对照组包括防止两种细胞类型之间直接接触的构建体。在使用新技术的支架种子中，碱性磷酸酶活性显著高于对照，体内牙齿形态发育类似于天然牙齿，并且每个支架中仅形成一个牙齿结构。这些结果表明，新的细胞接种技术可能是有用的再生牙齿的形态调控。(c)2006爱思唯尔有限公司保留所有权利。
Progress is being made toward regenerating teeth by seeding dissociated postnatal odontogenic cells onto scaffolds and implanting them in vivo, but tooth morphology remains difficult to control. In this study, we aimed to facilitate tooth regeneration using a novel technique to sequentially seed epithelial cells and mesenchymal cells so that they formed appropriate interactions in the scaffold. Dental epithelium and mesenchyme from porcine third molar teeth were enzymatically separated and dissociated into single cells. Mesenchymal cells were seeded onto the surface of the scaffold and epithelial cells were then plated on top so that the two cell types were in direct contact. The cell-scaffold constructs were evaluated in vitro and also implanted into immunocompromised rats for in vivo analysis. Control groups included constructs where direct contact between the two cell types was prevented. In scaffolds seed using the novel technique, alkaline phosphatase activity was significantly greater than controls, the tooth morphology in vivo was developed in similar to that of natural tooth, and only one tooth structure formed in each scaffold. These results suggest that the novel cell-seeding technique could be useful for regulating the morphology of regenerated teeth. (c) 2006 Elsevier Ltd. All rights reserved.