Characterization of the cutaneous inflammatory infiltrate in canine atopic dermatitis
Characterization of the cutaneous inflammatory infiltrate in canine atopic dermatitis
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DOI：
10.1097/00000372-199710000-00008
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发表时间：
1997-10-01
影响因子：
1.1
通讯作者：
Moore, PF
中科院分区：
文献类型：
--
作者：
Olivry, T;Naydan, DK;Moore, PF
通过光学显微镜和免疫过氧化物酶法，使用犬白细胞抗原特异性单克隆抗体，对病变特应性、临床正常特应性和正常犬皮肤切片进行了研究。我们证实，犬特应性皮炎的皮肤浸润细胞由肥大细胞、树突状抗原呈递细胞、记忆辅助性T淋巴细胞、少量嗜酸性粒细胞和中性粒细胞以及罕见的B淋巴细胞组成。表皮嗜酸性粒细胞微聚集体和聚集的朗格汉斯细胞的存在支持表皮过敏原接触的假设。表达γ/δ T细胞受体的表皮T细胞的增生似乎是犬特应性皮炎与人类特应性皮炎相比所特有的。这一发现可以通过皮肤免疫系统的种间差异来解释，或者通过这些亲上皮γ/δ T细胞在犬皮肤过敏性免疫应答中的积极参与来解释。犬特应性皮炎自发性病变中真皮中性粒细胞的缺乏与过敏性犬皮内变应原注射引起的嗜中性粒细胞丰富的晚期反应明显不同。这种细胞浸润的差异可能是由于单次和重复变应原接触以及表皮与真皮抗原接触之间的免疫反应差异所致。
Sections from lesional atopic, clinically normal atopic, and normal canine skin were investigated by light microscopy and an immunoperoxidase method using monoclonal antibodies specific for canine leukocyte antigens. We confirmed that skin-infiltrating cells of canine atopic dermatitis are constituted of mast cells, dendritic antigen-presenting cells, memory helper T-lymphocytes, low numbers of eosinophils and neutrophils, and rare B-lymphocytes. The presence of epidermal eosinophil microaggregates and clustered Langerhans' cells supports the hypothesis of epidermal allergen contact. The hyperplasia of epidermal T-cells expressing the gamma/delta T-cell receptor appears specific to canine atopic dermatitis compared with its human counterpart. This finding could be explained by an interspecies difference in skin immune systems or, alternatively, by an active participation of these epitheliotropic gamma/delta T-cells in the cutaneous allergic immune response in dogs. The paucity of dermal neutrophils in spontaneous lesions of canine atopic dermatitis is notably different from the neutrophil-rich late-phase reactions provoked by intradermal allergen injections in allergic dogs. This difference in the cellular infiltrate probably results from variations in the immune reaction between single and repeated allergen exposure as well as epidermal versus dermal antigen contact.