Successful pregnancies in cows following double freezing of a large volume of semen
Successful pregnancies in cows following double freezing of a large volume of semen
复制标题
DOI：
10.1051/rnd:2002044
复制
发表时间：
2002-11-01
期刊：
影响因子：
--
通讯作者：
Gacitua, H
中科院分区：
文献类型：
--
作者：
Arav, A;Zeron, Y;Gacitua, H
本文介绍了一种新的12mL试管大容量双冷冻精液技术。用一种新技术冷冻了两种不同公牛的精液，用12毫升试管冷冻，然后在迷你吸管解冻后重新冷冻。所有的冷冻都是在“多温度梯度”(MTG)冷冻设备中进行的，该设备以恒定的速度(V)通过温度梯度(G)来移动集装箱，从而产生受控的冷却速度B=(G)x(V)。两头射精的公牛都在实验室里被评估了解冻后的活动能力，然后在现场试验中进行了分裂抽样。我们对105头母牛进行了双重冷冻/解冻后的授精，并对123头母牛进行了微型吸管冷冻精液和常规方法的授精。结果表明，冷冻12mL试管后，解冻后的运动能达到75+/-5%，解冻2次后的运动能达到50+/-5%。受控蒸气冷冻显示解冻后60%+/-10%的运动。现场试验结果表明，复冻组妊娠率为44%(47/105)，对照组为45.5%(56/123)。这些结果对大体积冷冻是有益的，因此对公牛精液进行大体积冷冻是有益的，随后将在常规授精体积中进行二次冷冻。
The objective of the following paper is to describe a new technology for large volume and double freezing of semen in 12 mL test tubes. Semen from two different bulls was frozen with a new technique using 12 mL test tubes and was refrozen after thawing in mini straws. All freezing was done in a "Multi thermal gradient" (MTG) freezing apparatus, which moves the container at a constant velocity (V) through a thermal gradient (G) producing a controlled cooling rate B = (G) x (V). Each of the two bulls ejaculated were evaluated for post thaw motility in the lab and then in a field trial which was carried out in a split sample mode. We inseminated 105 cows after a double freezing/thawing cycle, and another 123 cows were inseminated with semen frozen in mini-straws and a conventional method. The results showed a 75 +/- 5% post thaw motility after freezing a 12 mL test tube and 50 +/- 5% after a second freezing/thawing in mini-straws, respectively. Controlled vapour freezing showed a 60 +/- 10% post thaw motility. The results of the field trial showed a pregnancy rate of 44% (47/105) for the double freezing group in comparison to 45.5% (56/123) for the controlled group. These results can be beneficial for large volume freezing, and therefore for bull semen cryobanking in a large volume which will be followed by second freezing in a regular insemination volume.