Effects of heavy water (D2O) on human pancreatic tumor cells.
Effects of heavy water (D2O) on human pancreatic tumor cells.
复制标题
DOI：
--
复制
发表时间：
2005-09
影响因子：
2
通讯作者：
J. Hartmann;Y. Bader;Z. Horváth;P. Saiko;M. Grusch;C. Illmer;S. Madlener;M. Fritzer-szekeres;Nicole Heller;R. Alken;T. Szekeres
中科院分区：
文献类型：
--
作者：
J. Hartmann;Y. Bader;Z. Horváth;P. Saiko;M. Grusch;C. Illmer;S. Madlener;M. Fritzer-szekeres;Nicole Heller;R. Alken;T. Szekeres
背景胰腺癌是一种难以治疗的疾病，因此大多数是致命的。由于重水（氧化氘，D2 O）在体外和体内的各种癌细胞系中显示出活性，我们现在研究其在人胰腺肿瘤细胞中的作用。材料与方法采用三种胰腺癌细胞系（AsPC-1、BxPC-3和PANC-1）检测D2 O的细胞毒作用。Hoechst/碘化丙啶双染色法检测细胞凋亡，流式细胞仪分析细胞周期分布。结果D2 O对AsPC-1、PANC-1和BxPC-3细胞的IC 50值分别为15%、18%和27%，并诱导细胞凋亡。此外，D2 O导致细胞周期停滞在G2-M期（BxPC-3，PANC-1）或S期（AsPC-1）。结论D2 O有望为胰腺癌的治疗提供新的选择。
BACKGROUND Pancreatic cancer constitutes an entity which is difficult to treat and, therefore, mostly fatal. Since heavy water (deuterium oxide, D2O) was shown to be active in various cancer cell lines in vitro and in vivo, we now investigated its effects in human pancreatic tumor cells. MATERIALS AND METHODS The cytotoxic effects of D2O were examined in three pancreatic cancer cell lines (AsPC-1, BxPC-3 and PANC-1). Induction of apoptosis was determined by Hoechst/propidium iodide double staining and cell cycle distribution was investigated by FACS analysis. RESULTS Employing a clonogenic assay, D2O yielded IC50 values of 15%, 18% and 27% in AsPC-1, PANC-1 and BxPC-3 cells, respectively, and led to the induction of apoptosis when compared to untreated controls. Moreover, D2O caused a cell cycle arrest in the G2-M-phase (BxPC-3, PANC-1) or in the S-phase (AsPC-1). CONCLUSION It is hoped that D2O might offer an additional option for the treatment of pancreatic carcinomas.