Antigen-independent expansion of CD28hi CD8 cells from aged mice: cytokine requirements and signal transduction pathways.
Antigen-independent expansion of CD28hi CD8 cells from aged mice: cytokine requirements and signal transduction pathways.
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老年小鼠 CD28hi CD8 细胞的抗原依赖性扩增：细胞因子需求和信号转导途径。
DOI：
10.1093/gerona/58.12.b1063
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Miller,RichardA
中科院分区：
文献类型：
--
作者：
Ortiz-Suárez,Anavelys;Miller,RichardA
来自年老小鼠的记忆 CD8+T 细胞可以在未受辐射的受体中增殖。将标记细胞从老年供体转移到年轻受体中表明，老年供体 CD8 细胞的增殖需要宿主细胞既能响应干扰素 γ 又能产生白细胞介素 15。从宿主小鼠中重新分离转移的 CD8 细胞表明，在从旧供体中恢复的 CD8 T 细胞中，无论它们是否在体内分裂，LAT（激活 T 细胞的连接子）易位到免疫突触和 NF（核因子）-κB 易位到细胞核的情况均减少。能够在体内增殖的细胞可以根据其异常高水平的 CD28 表达进行分离，但发现其与其他老化 CD8 细胞的 LAT 和蛋白激酶 C-theta (PKC-𝛉) 易位至免疫突触水平较低方面没有区别。因此，老年小鼠 CD28hiCD8 细胞的体内增殖不能归因于 T 细胞受体信号传导的保留。
Memory CD8+T cells from old mice can proliferate in nonirradiated recipients. Transfer of labeled cells from aged donors into young recipients showed that proliferation of aged donor CD8 cells requires host cells that can both respond to interferon-γ and produce interleukin-15. Reisolation of transferred CD8 cells from host mice showed that LAT (linker for activated T cells) translocation to the immunological synapse, and translocation of NF (nuclear factor)-κB to the nucleus were diminished in recovered CD8 T cells from old donors, whether they had divided in vivo or not. Cells able to proliferate in vivo could be isolated based on their unusually high levels of CD28 expression, but were found not to differ from other aged CD8 cells in their low levels of LAT and protein kinase C-theta (PKC-𝛉) translocation to the immunological synapse. Thus in vivo proliferation of CD28hiCD8 cells from aged mice cannot be attributed to retention of T-cell receptor signaling.