Preventive Effects of Mallotus japonicus Cortex Extracts on Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57 BL/6J Mice
Preventive Effects of Mallotus japonicus Cortex Extracts on Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57 BL/6J Mice
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DOI：
10.2174/2210315509666191106112622
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发表时间：
2020-03
期刊：
影响因子：
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通讯作者：
Y. Kimura
中科院分区：
文献类型：
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作者：
Y. Kimura
在日本，大戟属植物的皮层传统上被用来治疗胃溃疡、十二指肠溃疡和胃酸过多。近年来，大量研究集中于其对炎症性肠病(IBD)的影响。本研究的目的是利用炎症性肠病(IBD)小鼠模型研究日本血吸虫提取物(MJ)对大肠腹泻和炎症的影响。本研究使用3%葡聚糖硫酸钠(DSS)治疗结肠炎模型。在实验期间，除红细胞压积(Ht)、血红蛋白(Hb)、结肠细胞因子和趋化因子水平外，还检测了经DSS处理的C57BL/6J小鼠的红细胞、血小板和白细胞计数。口服MJ(200 mg/kg和500 mg/kg)的3%DSS组小鼠的疾病活动指数(DAI)低于单纯口服3%DSS组。此外，MJ还抑制了DSS处理小鼠的红细胞和血小板计数以及Hb和Ht水平的下降。结肠组织学直接快速猩红染色显示，MJ可防止DSS治疗所致的粘膜溃疡和嗜酸性粒细胞的渗入。灌胃给药组小鼠结肠单核细胞趋化蛋白-1、白介素1-β和肿瘤坏死因子-α水平升高。本研究结果提示，海参皮提取物通过抑制小鼠结肠IL-1β、肿瘤坏死因子-α和单核细胞趋化蛋白-1水平的升高以及结肠嗜酸性粒细胞的浸润，对肠易激疾病有有效的治疗作用。
The cortex of Mallotus japonicus (Euphorbiaceae) has traditionally been used to treat gastric ulcers, duodenal ulcers, and gastric hyperacidity in Japan. A large number of studies have recently focused on its effects on Inflammatory Bowel Disease (IBD). The aim of the present study was to examine the effects of M. japonicus (MJ) extracts on large intestinal diarrhea and inflammation using Inflammatory Bowel Disease (IBD) mouse models. The present study used 3% Dextran Sulfate Sodium (DSS)-treated colitis models. Red blood cell, platelet, and leukocyte counts in addition to hematocrit (Ht), hemoglobin (Hb), and colonic cytokine and chemokine levels were measured in DSS-treated C57BL/6J mice during the experimental period. The Disease Activity Index (DAI) was lower in 3% DSS-treated mice orally administered MJ (200 and 500 mg/kg) than in mice administered 3% DSS only. Furthermore, MJ inhibited decreases in red blood cell and platelet counts as well as Hb and Ht levels in DSS-treated mice. Colon histology using direct fast scarlet staining revealed that MJ prevented mucosal membrane ulceration and eosinophil infiltration of the mucosal membrane induced by the DSS treatment. Increases in colonic Monocyte Chemoattractant Protein 1 (MCP)-1, interleukin (IL)-1β, and Tumor Necrosis Factor (TNF)-α levels in DSS-treated mice were reduced by orally administered MJ extracts. The present results suggest that M. japonicus cortex extracts are an effective treatment for IBD through the inhibition of increases in colonic IL-1β, TNF-α, and MCP-1 levels and eosinophil infiltration of the colon in DSS-treated mice.