The gut microbiota contributes to the development of Staphylococcus aureus-induced mastitis in mice
The gut microbiota contributes to the development of Staphylococcus aureus-induced mastitis in mice
复制标题
肠道微生物群有助于金黄色葡萄球菌引起的小鼠乳腺炎的发生
DOI：
10.1038/s41396-020-0651-1
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发表时间：
2020-04-27
期刊：
影响因子：
11
通讯作者：
Zhang, Naisheng
中科院分区：
文献类型：
--
作者：
Hu, Xiaoyu;Guo, Jian;Zhang, Naisheng
乳腺炎是世界范围内奶牛养殖中最常见的疾病之一。肠道微生物群在全身和局部炎症性疾病（如乳腺炎）的调节中起着重要作用。然而，肠道微生物群对乳腺炎的调节机制仍不清楚。因此，本研究的目的是探讨肠道微生物群在宿主防御金黄色葡萄球菌（S。金黄色葡萄球菌）感染。血乳屏障通透性增加，S。金黄色葡萄球菌诱导的乳腺炎的严重程度观察肠道微生物群-生态失调小鼠与对照小鼠相比。此外，将粪便微生物群移植（FMT）到微生物群-生态失调小鼠中逆转了这些效应。此外，已建立的体内平衡的破坏导致致病性肠杆菌属细菌的丰度显著增加，而产生短链脂肪酸（SCFA）的细菌门（厚壁菌门和拟杆菌门）的相对丰度显著降低。然而，FMT肠道微生物群失调小鼠逆转了这些变化。此外，微生态失调降低了SCFAs的水平，丙酸钠、丁酸钠和益生菌（产丁酸菌）的给药逆转了血乳屏障的变化，降低了由S.金黄色。总之，这一新发现表明，肠道微生物群在宿主防御乳腺炎中起保护性作用，靶向肠道-乳腺轴代表了乳腺炎治疗的一种有前途的治疗方法。
Mastitis is one of the most prevalent diseases in dairy farming worldwide. The gut microbiota plays an important role in the regulation of systemic and local inflammatory diseases, such as mastitis. However, the regulatory mechanism of the gut microbiota on mastitis is still unclear. Thus, the aim of this study was to investigate the function and regulatory mechanisms of the gut microbiota in host defense against mastitis caused by Staphylococcus aureus (S. aureus) infection. Increased blood-milk barrier permeability, and S. aureus-induced mastitis severity were observed gut microbiota-dysbiosis mice compared with those in control mice. Moreover, feces microbiota transplantation (FMT) to microbbiota-dysbiosis mice reversed these effects. Furthermore, established disruption of commensal homeostasis results in significantly increased abundance of pathogenic Enterobacter bacteria, while the relative abundance of short-chain fatty acid (SCFAs)-producing bacterial phyla (Firmicutes and Bacteroidetes) was significantly reduced. However, FMT to gut microbiota-dysbiosis mice reversed these changes. In addition, dysbiosis reduced the levels of SCFAs, and administration of sodium propionate, sodium butyrate, and probiotics (butyrate-producing bacteria) reversed the changes in the blood-milk barrier and reduced the severity of mastitis induced by S. aureus. In conclusion, this new finding demonstrated that the gut microbiota acts as a protective factor in host defense against mastitis and that targeting the gut-mammary gland axis represents a promising therapeutic approach for mastitis treatment.